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Recombinant isoamylase

Manufactured by Merck Group
Sourced in United States

Recombinant isoamylase is a laboratory enzyme. It catalyzes the hydrolysis of internal α-1,6-glycosidic linkages in amylopectin and other polysaccharides.

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2 protocols using recombinant isoamylase

1

Fibroblast Proteomics and Metabolism

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High-performance liquid chromatography-grade acetonitrile, ethanol, methanol, water, trifluoroacetic acid (TFA), bleomycin (B5507, Lot#SLCG3757), and recombinant isoamylase were purchased from Sigma-Aldrich. α-cyano-4-hydroxycinnamic acid (CHCA) matrix was purchased from Cayman Chemical. Histological-grade xylenes were purchased from Spectrum Chemical. Citraconic anhydride for antigen retrieval was obtained from Thermo Fisher Scientific. Recombinant PNGaseF Prime was obtained from N-Zyme Scientifics (Doylestown, PA, USA). Normal lung fibroblast isolated from adult lung tissue and diseased lung fibroblast isolated from adult idiopathic pulmonary fibrosis lung tissue were purchased from Lonza, USA (Cat# CC-2512 and CC-7231, respectively). 13C6-glucose was purchased from Cambridge Isotope Laboratories, Inc (Cat# CLM-1396-pk).
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2

Proteomic Sample Preparation Protocol

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High-performance liquid chromatography-grade acetonitrile, ethanol, methanol, water, and trifluoroacetic acid (TFA) were purchased from Sigma-Aldrich. α-cyano-4-hydroxycinnamic acid (CHCA) matrix was purchased from Cayman Chemical. Histological-grade xylenes was purchased from Spectrum Chemical. Citraconic anhydride for antigen retrieval was obtained from Thermo Fisher Scientific. Recombinant PNGaseF Prime was obtained from N-Zyme Scientifics (Doylestown, PA, USA), and recombinant isoamylase was obtained from Sigma-Aldrich.
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