Ferrous sulfate 2

Potassium ferricyanide, ferrous sulfate (II), ethylenediaminetetraAcetic acid (EDTA), gallic acid, ammonium molybdate, hydrogen peroxide 30%, Acetic acid, Folin–Ciocalteu phenol reagent, ethanol, and sulfuric acid were obtained from Merck (Darmstadt, Germany). Nitro blue tetrazolium (NBT), monosaccharides, EDTA, ascorbic acid, methionine, bovine serum albumin (BSA), pyrocatechol violet, ascorbic acid, and ammonium molybdate were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). Sodium bicarbonate, culture media components (Minimum essential Dulbecco’s modified Eagle medium (DMEM)), non-essential amino acids, fetal bovine serum, sodium pyruvate, and phosphate buffered saline (PBS) were purchased from Invitrogen Canada Inc. (Burlington, ON, Canada). Acetonitrile (HPLC grade) purchased from Panreac^® (São Paulo, Brazil). Acetic acid was provided by Vetec^® (São Paulo, Brazil). Water was purified with a Milli-Q system (Millipore^®, Bedford, MA, USA). All other solvents and chemicals were of analytical grade.

Ethanol, mEthanol, sulfuric acid, potassium ferricyanide, ferrous sulfate (II), and aluminum chloride were obtained from Merck (Germany). Diphenylpicryl hydrazine (DPPH), catechin, and 3-(4,5-dimethylthiazol-2-yl)-2-5-diphenyltetrazolium bromide (MTT) were purchased from Sigma Chemical (USA). Sodium chloride was purchased from Sigma Chemical. HeLa cells (ATCC¯ CCL-2), SiHa cells ATCC¯ (HTB-35), 786-0 (ATCC¯ CRL-1932, HT-29 (ATCC¯ HTB-38), and Embryo fibroblast 3T3 (ATCC¯ CCL-164) were provided by Dr. Hugo Oliveira Rocha, Department of Biochemistry, UFRN, Brazil. Cell culture medium components (Dulbecco^'s Modified Eagle Medium, DMEM) and trypsin were obtained from Cultilab (Brazil). Sodium bicarbonate, sodium pyruvate and phosphate buffered saline (PBS) were purchased from Invitrogen Corporation (Canada). All other solvents and chemicals were of analytical grade.

Potassium ferricianyde, ferrous sulfate II, trichloroacetic acid, and sulfuric acid were purchased from Merck (Darmstadt, Germany). Nitro Blue Tetrazolium (NBT), monosaccharides, diaminoethanetetraacetic acid (EDTA), ascorbic acid, methionine, 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT), Griess reagent, pyrocatechol violet, riboflavin, and ammonium molybdate were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). Sodium bicarbonate, non-essential amino acids, and phosphate buffered saline (PBS) were purchased from Invitrogen Corporation (Burlington, ON, Canada). Dulbecco′s modified Eagle′s medium (DMEM) and fetal bovine serum (FBS) were obtained from CULTILAB (Campinas, SP, Brazil). Penicillin and streptomycin were obtained from Gibco (Fort Worth, TX, USA). All other solvents and chemicals were of analytical grade.
The HEK-293 cells (ATCC^® CRL-1573™) were grown in a DMEM medium with 10% FBS, 100 μg/mL streptomycin and 100 IU/mL Penicillin. The cells were cultured in DMEM supplemented with FBS (10% v/v) and antibiotics (100 U/mL Penicillin and 100 μg/mL streptomycin), at 37 °C in a humidified atmosphere with 5% CO₂. For maintenance of the cells, the culture medium was changed every three days, and the cells were further subcultured using trypsin/EDTA purchased from CULTILAB (Campinas, SP, Brazil).