Geldoc go image software

The Y-shaped DNA nanostructures (Y-DNA) with C-rich sticky ends were designed to be synthesized by CHA-mediated reactions of three individual hairpin DNAs (HDs) using NUPACK software (http://www.nupack.org). To prepare the Y-DNA, three HD solutions (HD1, HD2, and HD3) were heated to 94 °C for 5 min and then rapidly cooled to 10 °C using a thermal cycler (BIOER, China) to form stable hairpin nanostructures. The resultant HDs were mixed together in equal molar ratios and then incubated with the target DNA for 1 h at room temperature. The final products, Y-DNA, were verified by 8% polyacrylamide gel electrophoresis (PAGE) in TAE (Trizma base, acetic acid, and 0.5 M EDTA, at pH 8.0) buffer at 150 V for 40 min. The gel was stained with GelRed® (Biotium, USA) for 1 min and visualized using a GelDoc Go system (Bio-Rad, USA). The band intensities were quantitatively compared using GelDoc Go image software (Bio-Rad, USA).

Biotinylated DNA oligonucleotides (HD1 and HD2) were completely dissolved in 1X phosphate buffered saline (PBS, at pH 7.4). Each resultant product was heated to 94 °C for 5 min and then rapidly cooled to 10 °C using a thermal cycler (BIOER, China) to form stable hairpin DNA nanostructures. To amplify dimeric DNA probes, target cDNA was added to a mixture of HD1 and HD2 and then incubated for different periods of time at room temperature. The resultant products, dimeric DNA probes, were verified using 12% PAGE analysis. The band intensity was quantitatively compared using GelDoc Go image software (Bio-Rad, USA).