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Cyclin b2

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Cyclin B2 is a protein that plays a crucial role in the regulation of the cell cycle. It is a member of the cyclin family and is involved in the transition from the G2 phase to the M phase of the cell cycle. Cyclin B2 binds to and activates the cyclin-dependent kinase, CDC2, which is essential for the initiation of mitosis.

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Lab products found in correlation

Ythdf2, by Proteintech (1 mentions) Peroxidase conjugated affinipure goat anti mouse igg h l, by Proteintech (1 mentions) Ythdf3, by Proteintech (1 mentions) P21 10355 1 ap, by Proteintech (1 mentions) Brca1, by Proteintech (1 mentions) Ythdf1, by Proteintech (1 mentions) Flag tag (flag), by Merck Group (1 mentions) Gapdh 60004 1 ig, by Proteintech (1 mentions) α tubulin, by Proteintech (1 mentions) Mettl3, by Proteintech (1 mentions) Rabbit igg, by Proteintech (1 mentions) Cyclin b1, by Cell Signaling Technology (1 mentions) Nanog, by Cell Signaling Technology (1 mentions) Tgfbr1, by Abcam (1 mentions) Phosphorylated smad2, by Cell Signaling Technology (1 mentions) Smad2, by Cell Signaling Technology (1 mentions)

2 protocols using cyclin b2

1

Western Blot Analysis of DNA Damage Markers

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Protein extracts were resolved by sodium dodecyl sulfate-polyacrylamide gel, transferred to polyvinylidene fluoride membranes, and probed with antibodies against ATM (2873S, Cell Signaling Technology, CST); p-ATM -S1981 (AP0008, ABclonal); METTL3 (15073-1-AP, Proteintech); YTHDF1 (17479-1-AP, Proteintech); YTHDF2 (24744-1-AP, Proteintech); YTHDF3 (25537-1-AP, Proteintech); FTO (TA809392, OriGene); m6A (202003, Synaptic Systems); BRCA1 (20649-1-AP, Proteintech); H2A.X (A11361, ABclonal); γH2A.X (AP0687, ABclonal ); GAPDH(60004-1-Ig, Proteintech); eIF3A (3411T, CST); Flag (F1804, Sigma-Aldrich); anti‐Rabbit IgG HRP‐linked antibody (7074; CST); Peroxidase-conjugated Affinipure Goat Anti-Mouse IgG (H+L) (SA00001-1, Proteintech); α-Tubulin (66031-1-Ig, Proteintech); CHK1(bs-1681R, Bioss); CHK2 (252092, Zenbio); p-CHK2-S19(AP0862, ABclonal); Anti-HA tag monoclonal antibody (TA100012, OriGene); Mouse-IgG (BA1046, Boster); Rabbit IgG (B900610, Proteintech); p21(10355-1-AP, Proteintech); 53BP1 (BA2878, Boster); CDK1 (D160158, BBI life sciences); CDK2 (D199431, BBI life sciences); Cyclin B2 (21644-1-AP, Proteintech).
Zhang X., Liu P., Zheng X., Wang J., Peng Q., Li Z., Wei L., Liu C., Wu Y., Wen Y., Yan Q, & Ma J. (2021). N6-methyladenosine regulates ATM expression and downstream signaling. Journal of Cancer, 12(23), 7041-7051.
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2

Western Blotting Analysis of Stem Cell Markers

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Western blotting was performed, as described previously [41 (link)], using antibodies against ALG3 (NO. 20290–1-AP, PROTEINTEC, Manchester, UK), TGFBR2(NO. 79424, Cell Signaling Technology, Inc., Beverly, Massachusetts, USA), TGFBR1(NO. ab31031, Abcam, Inc., Cambridge science park, UK), phosphorylated SMAD2 (NO. 55041, Cell Signaling Technology, Inc., Beverly, Massachusetts, USA), SMAD2 (NO. 5339, Cell Signaling Technology, Inc., Beverly, Massachusetts, USA), NANOG (NO. 3580, Cell Signaling Technology, Inc., Beverly, Massachusetts, USA), SOX2 (NO. 4900, Cell Signaling Technology, Inc., Beverly, Massachusetts, USA), OCT4 (NO.2750, Cell Signaling Technology, Inc., Beverly, Massachusetts, USA), CyclinB1 (NO. 4135, Cell Signaling Technology, Inc., Beverly, Massachusetts, USA), CyclinB2 (NO. 21644–1-AP, Proteintech Group, Inc., Rosemont, IL, USA) and CDK4 (NO. 12790, Cell Signaling Technology, Inc., Beverly, Massachusetts, USA). Anti-α-Tubulin mouse monoclonal antibody (NO. 66031–1-Ig, Proteintech Group, Inc., Rosemont, IL, USA) was used to confirm equal loading.
Sun X., He Z., Guo L., Wang C., Lin C., Ye L., Wang X., Li Y., Yang M., Liu S., Hua X., Wen W., Lin C., Long Z., Zhang W., Li H., Jian Y., Zhu Z., Wu X, & Lin H. (2021). ALG3 contributes to stemness and radioresistance through regulating glycosylation of TGF-β receptor II in breast cancer. Journal of Experimental & Clinical Cancer Research : CR, 40, 149.
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