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Atp disodium salt ia0590

Manufactured by Solarbio

ATP (disodium salt) (IA0590) is a laboratory reagent that is commonly used as an energy source in various biochemical processes. It serves as the primary carrier of energy in living cells and plays a crucial role in cellular metabolism.

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2 protocols using atp disodium salt ia0590

1

Receptor Antagonist and Signaling Protein Assay

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AMD3100 (S8030, Plerixafor, antagonist against CXCR4) was purchased from Selleckchem. Poly-D-lysine (C0312) was purchased from Beyotime Biotechnology. ATP (disodium salt) (IA0590) was purchased from Solarbio.
Recombinant human Src protein (ab79635, His tag active Src) was purchased from Abcam. Recombinant human/feline/rhesus macaque (350-NS) was purchased from R&D systems.
Antibody to human CXCR4 (ab181020) and anti-GFP antibody (ab6556) were purchased from Abcam. Antibodies to RhoGDI2 (sc-271042) and ABL1 (sc-56887) were purchased from Santa Cruz biotechnology. Anti-RhoGDI2 (D262966) for Western blot assay, anti-ACTB (D110001), anti-Src antibody (D221267), and anti-GST-Tag pAb (AE006) were purchased from Sangon Biotech Company. Rabbit mAb to phospho-Src family (Tyr416) (D49G4), rabbit mAb to RhoA (67B9), and rabbit mAb to RhoC (D40E4) were purchased from Cell Signaling Technology. Rabbit mAb to phospho-Lck family (Tyr394) (D155064) was purchased Sangon Biotech. Mouse anti-His-Tag mAb (AE003) was purchased from ABclonal. Monoclonal antibody to phosphotyrosine produced in mouse (PY20, P4110) was purchased from Sigma-Aldrich. Fast Mutagenesis System (K11209) was purchased from TransGen Biotech.
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2

Proteomic Analysis of Protein Interactions

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Recombinant Human/Feline/RhesusMacaque (Cat. no. 350-NS) and Recombinant Mouse CXCL12/SDF-1α (Cat. no.460-SD) were purchased from R&D systems. Poly-D-lysine (C0312) was purchased from Beyotime Biotechnology. ATP (disodiumsalt) (IA0590) was purchased from Solarbio.
Monoclonal antibody (mAb) against ABL1(sc-56887) and monoclonal antibody (mAb) against Cofilin1(E-8) (sc-376476) were purchased from Santa Cruz. Monoclonal antibody to phosphotyrosine produced in mouse (PY20, P4110) was purchased from Sigma-Aldrich. Antibody against ACTB (D110001) was purchased from Sangon Biotech Company in China. Antibodies against GST-Tag (AE006) and His-tag (AE003) were purchased from Abclonal. Ammonium bicarbonate, dithiothreitol (DTT), iodoacetamide (IAA), and sodium carbonate were purchased from Sigma-Aldrich (St. Louis, MO). Urea and Sodium dodecyl sulfate (SDS) were purchased from Bio-Rad (Hercules, CA).
Acetonitrile and water for nano-LC-MS/MS were purchased from J. T. Baker (Phillipsburg, NJ).
Trypsin was purchased from Promega (Madison, WI). All other chemical reagents were purchased at analytical grade.
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