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2 oligodeoxynucleotides

Manufactured by Integrated DNA Technologies
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2'-oligodeoxynucleotides are short synthetic DNA sequences typically ranging from 15 to 25 nucleotides in length. They are used as building blocks in various molecular biology applications, such as gene synthesis, antisense technology, and as primers for DNA amplification.

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Lab products found in correlation

Proteinase k, by Thermo Fisher Scientific (6 mentions) T4 ligase, by Thermo Fisher Scientific (3 mentions) T4 kinase, by Thermo Fisher Scientific (3 mentions) Puc19 plasmid and restriction enzymes, by New England Biolabs (2 mentions) Restriction enzyme, by New England Biolabs (1 mentions)

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Oligodeoxynucleotides

4 protocols using 2 oligodeoxynucleotides

1

Plasmid Isolation and Manipulation

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The pUC19 plasmid and restriction enzymes were obtained from New England Biolabs (Ipswich, MA). The T4-kinase, T4 ligase and proteinase K were purchased from ThermoFisher Scientific; E. coli DNA gyrase was from Millipore Sigma. The 2′-oligodeoxynucleotides were purchased from Integrated DNA Technologies (Coralville, IA, USA) and purified by denaturing polyacrylamide gel electrophoresis.
Kolbanovskiy M., Aharonoff A., Sales A.H., Geacintov N.E, & Shafirovich V. (2021). Base and Nucleotide Excision Repair Pathways in DNA Plasmids Harboring Oxidatively Generated Guanine Lesions. Chemical research in toxicology, 34(1), 154-160.
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2

Plasmid Manipulation and Oligonucleotide Purification

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The plasmid pUC19 and restriction enzymes were obtained from New England Biolabs (Ipswich, MA). The T4-kinase, T4 ligase, and proteinase K were purchased from ThermoFisher Scientific. The 2′-oligodeoxynucleotides were obtained from Integrated DNA Technologies (Coralville, IA) and purified by denaturing polyacrylamide gel electrophoresis before use.
Kolbanovskiy M., Aharonoff A., Sales A.H., Geacintov N.E, & Shafirovich V. (2020). Remarkable Enhancement of Nucleotide Excision Repair of a Bulky Guanine Lesion in a Covalently Closed Circular DNA Plasmid Relative to the Same Linearized Plasmid. Biochemistry, 59(31), 2842-2848.
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3

Cloning and Characterization of pUC19

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The pUC19 plasmid and restriction enzymes were obtained from New England Biolabs (Ipswich, MA); The T4-kinase, T4 ligase and proteinase K were purchased from ThermoFisher Scientific. The 2′-oligodeoxynucleotides were purchased from Integrated DNA Technologies (Coralville, IA, USA) and purified by denaturing polyacrylamide gel electrophoresis.
Kolbanovskiy M., Aharonoff A., Sales A.H., Geacintov N.E, & Shafirovich V. (2021). Recognition and Repair of Oxidatively Generated DNA Lesions in Plasmid DNA by a Facilitated Diffusion Mechanism. The Biochemical journal, 478(12), 2359-2370.
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4

Purification and Utilization of RecQ Helicase

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Recombinant E. coli RecQ helicase was purchased from the company Abcam. The 2′-oligodeoxynucleotides were purchased from Integrated DNA Technologies (Coralville, IA, USA). The racemic anti-BPDE ((+/−)-7R,8S)-dihydroxy-(9S,10R)-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene, known as (+)-or (−)-anti-BPDE) was originally obtained from the National Cancer Institute Chemical Carcinogen Repository.
Sales A.H., Zheng V., Kenawy M.A., Kakembo M., Zhang L., Shafirovich V., Broyde S, & Geacintov N.E. (2022). Inhibition of E. coli RecQ Helicase Activity by Structurally Distinct DNA Lesions: Structure—Function Relationships. International Journal of Molecular Sciences, 23(24), 15654.
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