Cd32 pe

Manufactured by BD

Sourced in United States

The CD32-PE is a laboratory instrument used for the detection and analysis of specific cellular markers. It employs flow cytometry technology to identify and quantify the expression of the CD32 protein on the surface of cells. The CD32-PE provides researchers with a precise and reliable tool for immunophenotyping and cellular characterization studies.

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Lab products found in correlation

Cd64 pe, by BD (1 mentions) Cd11b apc cy7, by Thermo Fisher Scientific (1 mentions) Goat anti rabbit pe, by Abcam (1 mentions) Facsdiva v6, by BD (1 mentions) Cd16 af700, by BD (1 mentions) 7 aad, by BD (1 mentions) Ve cadherin fitc, by BD (1 mentions) Cd43 pe, by BD (1 mentions) Facscalibur, by BD (1 mentions) Cd45 apc, by BD (1 mentions) Cd86 pe, by BD (1 mentions) Mhc 2 pe, by BD (1 mentions)

3 protocols using cd32 pe

Phenotyping Monocyte Subsets via Flow Cytometry

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Frozen PBMCs were thawed and distributed into three tubes for antibody staining. All tubes contained antibodies to the following markers: CD14-FITC (Southern Biotechnology Associates), CD16-AF700 (BD Bioscience), and CD3- and CD19-PerCP.Cy5.5 (eBioscience). Additional antibodies included: Tube 1, CD11b-APC-Cy7 (eBioscience) and CD32-PE (BD Bioscience); Tube 2, CD64-PE (BD Bioscience); and Tube 3, CD32B-PE detected with goat anti-rabbit PE (AbCAM). Proper isotype controls were included for each primary antibody. All incubations were done in ice. Fc-receptors were blocked with human IgG (Sigma-Aldrich) for 20 min, followed by addition of antibodies for 30 min in PBS with 1% bovine serum albumin, 0.1 mM EDTA and 0.02% sodium azide. Prior to analysis 7AAD (BD Bioscience) was added to each tube. Acquisition was conducted in a FACS CANTO-II using FACS DIVA v6.0 (BD Biosciences). Monocytes were identified based on their forward and side scatter properties and then dead (7AAD-positive), CD3 and CD19-positive cells were excluded. Further details on the gating strategy are provided in the Supplement (Fig. S2). After identifying monocyte sub-populations based on CD14 and CD16 expression, the median fluorescence intensities of each fluorochrome was established.

Restrepo B.I., Twahirwa M., Rahbar M.H, & Schlesinger L.S. (2014). Phagocytosis via Complement or Fc-Gamma Receptors Is Compromised in Monocytes from Type 2 Diabetes Patients with Chronic Hyperglycemia. PLoS ONE, 9(3), e92977.

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Flow Cytometric Analysis of Cell Markers

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Flow cytometric analysis was performed on Guava-easyCyte (6HT) and BD FACSCalibur flow cytometry systems, using InCyte or FlowJo software for data acquisition and analysis. The antibodies for staining human cell surface markers VE-cadherin-FITC, CD43-PE, CD45-APC and CD32-PE were obtained from BD Biosciences, NJ, USA.

Elcheva I., Sneed M., Frazee S., Liu Z., Zhu J., Wood T., Hendrickson S., Oehler C., Garcia B, & Spiegelman V.S. (2019). A novel hiPSC-derived system for hematoendothelial and myeloid blood toxicity screens identifies compounds promoting and inhibiting endothelial-to-hematopoietic transition. Toxicology in vitro : an international journal published in association with BIBRA, 61, 104622.

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Microglia Phenotyping by Flow Cytometry

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Isolated microglia were suspended in an incubation buffer (50 μl; 1 × PBS + 0.1%BSA) for 30 min on ice then F_c receptors blocked with anti-CD32 (BD Bioscience, San Jose, CA). Fluorescent conjugated antibodies were applied on ice for 30 min in the dark to assess microglia purity (mouse anti-rat CD11b-FITC, BD Pharmingen, San Jose, CA; mouse anti-rat-CD45-APC, eBioscience, San Diego, CA) and state of activation (mouse anti-rat: MHC-II-PE, CD32-PE, CD86-PE; BD Bioscience, San Jose, CA). For CD206, cells were incubated in rabbit anti-rat CD206 then donkey anti-rabbit-PE secondary antibody (BD Bioscience, San Jose, CA). Following washes in PBS, cells were analyzed on an Attune Acoustic Focusing Cytometer (ABI, Carlsbad, CA) calibrated with commercially available beads prior to each run. Fluorescence spillover compensation values were generated from non-stained cell populations and single-color staining controls. Isotype controls were used to exclude the non-specific binding of antibodies. For each staining condition, 1 × 10⁴ events were collected.

Peng H, & Nixon K. (2021). Microglia Phenotypes Following the Induction of Alcohol Dependence in Adolescent Rats. Alcoholism, clinical and experimental research, 45(1), 105-116.

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