The selected commercial drug products were purchased from a public pharmacy (Table 1 ). Hard gelatin capsules size 4 (Capsula GmbH, Ratingen, Germany) were used for those formulations, which represented pellets or powders. Tablet formulations were dose proportionally cut with a knife to create the respective in vitro dose (Table 1 ). The buffer concentrate contained tri-potassium phosphate (Alfa Aesar, Kandel, Germany), tri-potassium citrate (Carl Roth, Karlsruhe, Germany), and sodium hydroxide (VWR Chemicals, Darmstadt, Germany). Sodium-taurocholate, lecithin and 1-decanol were purchased from Alfa Aesar (Kandel, Germany). The high performance liquid chromatography HPLC chemicals consisted of methanol (VWR Chemicals, Darmstadt, Germany), and demineralized water (Merck Milli-Q, Darmstadt, Germany). 0.1 N hydrochloric acid was purchased from VWR Chemicals (Darmstadt, Germany). The following drug substances were used: aprepitant (TCI Deutschland, Eschborn, Germany), celecoxib (Acros Organics, Thermo Fisher Scientific, Geel, Belgium), itraconazole (Acros Organics, Thermo Fisher Scientific, Geel, Belgium), nimodipine (Alphar aesar, Landau, Germany) and ritonavir (AbbVie Deutschland GmbH & Co. KG, Ludwigshafen, Germany).
1 decanol
Manufactured by Thermo Fisher Scientific
Sourced in Germany
1-decanol is a long-chain primary alcohol with the chemical formula CH3(CH2)8CH2OH. It is a clear, colorless liquid with a mild odor. 1-decanol is commonly used as a solvent, chemical intermediate, and in the synthesis of various compounds.
Automatically generated - may contain errors
Lab products found in correlation
Lecithin, by Thermo Fisher Scientific (4 mentions)
Sodium taurocholate, by Thermo Fisher Scientific (4 mentions)
Tri potassium citrate, by Carl Roth (3 mentions)
Sodium hydroxide (naoh), by Avantor (2 mentions)
Methanol, by Avantor (2 mentions)
Celecoxib, by Thermo Fisher Scientific (1 mentions)
Itraconazole, by Thermo Fisher Scientific (1 mentions)
Cellulose acetate syringe filter, by Avantor (1 mentions)
Acetonitrile, by Avantor (1 mentions)
Ritonavir, by Abbvie (1 mentions)
Trifluoroacetic acid, by Merck Group (1 mentions)
Cytochrome c, by Thermo Fisher Scientific (1 mentions)
Tetrahydrofuran, by Merck Group (1 mentions)
Glycidyl methacrylate, by Merck Group (1 mentions)
Benzophenone, by Thermo Fisher Scientific (1 mentions)
1 propanol, by Merck Group (1 mentions)
1 4 butanediol, by Merck Group (1 mentions)
Hplc grade acetonitrile acn, by Merck Group (1 mentions)
Ethylene glycol dimethacrylate, by Merck Group (1 mentions)
Methanol meoh, by Merck Group (1 mentions)
5 protocols using 1 decanol
1
In Vitro Dissolution of Oral Dosage Forms
2
Ritonavir Amorphous Solid Dispersion Characterization
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Neat ritonavir and ritonavir-ASD were provided by AbbVie Germany, Ludwigshafen, Germany. The ritonavir-ASD was prepared as extrudate beads by hot melt extrusion on a co-rotating twin-screw extruder, and composed of ritonavir (15%), copovidone (74%), sorbitan monolaurate (10%), and colloidal silicon dioxide (1%). The purity of the ingredients was according to the compendial specifications (Ph.Eur./USP). The absence of API-related crystallinity in the ASD was confirmed by polarized light microscopy (Model DMLM, Leica Microsystems, Wetzlar, Germany). Tri-potassium phosphate (Alfa Aesar, Kandel, Germany), tri-potassium citrate (Carl Roth, Karlsruhe, Germany), and sodium hydroxide (VWR Chemicals, Darmstadt, Germany) were used for the buffer concentrate. Lecithin, 1-decanol, and sodium taurocholate were obtained from Alfa Aesar (Kandel, Germany). Glitter powder was purchased from Jofrika Cosmetics, Bergisch-Gladbach, Germany. The high performance liquid chromatography HPLC chemicals consisted of methanol (VWR Chemicals, Darmstadt, Germany), acetonitrile (VWR Chemicals, Darmstadt, Germany), and demineralized water (Merck Milli-Q, Darmstadt, Germany). Samples for HPLC were filtered through a 0.45 µm cellulose acetate syringe filter (VWR Chemicals, Darmstadt, Germany).
3
Synthesis and Characterization of Polymeric Stationary Phases
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Glycidyl methacrylate (GMA), butyl methacrylate (BMA), ethyleneglycol dimethacrylate (EDMA), tetrahydrofuran (THF), 1,4-butanediol, 1-propanol and trifluoroacetic acid (TFA) were from Sigma-Aldrich (Steinheim, Germany). Styrene (STY), dininylbenzene (DVB) (technical grade, 80% mixture of isomers, 20% mainly ethylstyrene), 1-decanol, 1-dodecanol, benzophenone (BP) and lauroyl peroxide (LPO) were supplied by Alfa Aesar (Karlsruhe, Germany). Azobisisobutyronitrile (AIBN) was from Fluka (Buchs, Switzerland). HPLC-grade acetonitrile (ACN) and methanol (MeOH) were from Merck (Darmstadt, Germany). Uracil, alkyl benzenes from Riedel de Haën (Seelze, Germany) and proteins such as ribonuclease A (bovine heart), cytochrome C (bovine pancreas) from Alfa Aesar, and myoglobin (horse skeletal muscle) from Sigma were used as probes. Acetone was supplied by Panreac (Barcelona, Spain). Ultra-pure water was obtained with a Puranity TU6 water purification system from VWR (Bedford, MA, USA) provided with a 0. Stock solutions of alkyl benzenes were prepared in ACN at 1.0 mg mL -1 each and kept at 4ºC until their use. Working standard solutions were freshly prepared by dilution to the desired concentration with the mobile phase. Proteins were dissolved in water at concentration of 1.0 mg mL -1 each and kept at -18ºC.
4
Itraconazole Metabolite Quantification Protocol
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The model drug itraconazole (ITZ) was obtained from Sris Pharmaceuticals (Hyderabad, India). Hydroxy-itraconazole (OH-ITZ, 0.1 mg/ml methanol solution), the major active metabolite of ITZ in rats (Yoo et al., 2000 (link)) as well as in humans (Heykants et al., 1989 (link)), was obtained from Sigma-Aldrich (Taufkirchen, Germany). Ketoconazole (KTZ), the internal standard, was obtained from ThermoFisher (Kandel, Germany). Sporanox® 100 mg capsules were obtained from Janssen GmbH (Neuss, Germany). HPC-SL (MW = 100,000 g/mol; 73.5% Hydroxypropoxy groups), -SSL (MW = 40,000 g/mol; 73.7% Hydroxypropoxy groups) and -UL (MW = 20,000 g/mol; 70.1% Hydroxypropoxy groups) were kindly donated by Nippon Soda Co., Ltd. (Tokyo, Japan). Affinisol® HME15LV (HPMC) was donated from Dow Chemical Company, (Bomlitz, Germany). LC-MS grade water was obtained from Bernd Kraft GmbH (Duisburg, Germany). LC-MS grade acetonitrile (AcN), potassium citrate monohydrate (> 99%) were obtained from VWR Chemicals GmbH (Darmstadt, Germany). Lecithin, sodium taurocholate, 1-decanol and potassium phosphate were obtained from ThermoFisher (Kandel, Germany). Sodium hydroxide was obtained from Honeywell Fluka (Seelze, Germany). 0.1 N HCl solutions were obtained from VWR Chemicals (Fontenay-sous-Bois, France). Dimethyl sulfoxide (DMSO, > 99.9%) was obtained from Fisher Scientific (Geel, Belgium).
5
Formulation and Characterization of Lipid Nanocarriers
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CorA was provided by the Helmholtz Centre for Infection Research (Braunschweig, Germany). Polyvinylpyrrolidone (Kollidon® 30 LP) and vinylpyrrolidone-vinyl acetate copolymer (Kollidon® VA 64) were kindly provided by BASF SE (Ludwigshafen, Germany). Ethanol 99.8% and tri-potassium citrate were purchased from Carl Roth GmbH & Co. KG (Karlsruhe, Germany). Tri-potassium phosphate, lecithin, 1-decanol and sodium taurocholate were purchased from Thermo Fisher GmbH (Kandel, Germany). Sodium oleate was purchased from Sigma (Darmstadt, Germany). Glycerol mono-oleate was kindly provided by Gattefosseé SAS (Saint-Priest, France). In addition, 1-micron full-flow polyethylene filters were purchased from Cole-Parmer GmbH. Acetic acid was purchased by Sigma Aldrich Chemie GmbH (Steinheim, Germany). Sodium hydroxide was purchased from VWR International, LLC (Darmstadt, Germany). Water for injection was purchased from Fresenius Kabi Deutschland GmbH (Bad Homburg, Germany). LC-MS grade acetonitrile and water were purchased from Bernd Kraft GmbH (Duisburg, Germany), and LC-MS grade ammonium acetate from Merck KGaA (Darmstadt, Germany). Cell culture reagents were purchased from Sigma Aldrich (Taufkirchen, Germany).
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