Rabbit anti mtor

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Rabbit anti-mTOR is a primary antibody that specifically binds to the mammalian target of rapamycin (mTOR) protein. mTOR is a serine/threonine protein kinase that plays a crucial role in the regulation of cell growth, proliferation, and metabolism. This antibody can be used for the detection and quantification of mTOR in various research applications.

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3 protocols using rabbit anti mtor

Hippocampal mTOR Pathway in Chronic Cerebral Hypoperfusion

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To examine changes in mTOR and p-mTOR protein levels in the hippocampal CA1 region following CCH, the sham- and CCH-operated animals (n = 5 each at 7, 14, 21, and 28 days after surgery) were used for western blot analysis performed according to the method described in our previous studies [14 (link)15 (link)19 (link)]. In brief, after sacrificing the animal and removing the brain, the brain was serially and transversely cut into 400 µm thick sections on a vibratome (VP1000P; Leica), and the hippocampal CA1 regions were then dissected with a surgical blade. The tissues were homogenized and centrifuged, and the obtained supernatants were subjected to western blot analysis. Rabbit anti-mTOR (1:200; Santa Cruz Biotechnology), rabbit anti-p-mTOR (1:200; Santa Cruz Biotechnology), or mouse anti-β-actin (1:5,000; Sigma) was used as the primary antibody. Western blot analysis was performed with three repetitions. After scanning the western blotting results, densitometric analysis of western blot bands was performed by using Image J 1.46 software (National Institutes of Health, USA), to measure relative optical density (ROD). Ratios of the ROD of CCH-operated groups were represented as percentage values, with the sham-group results designated as 100%.

Park J.A, & Lee C.H. (2017). Temporal changes in mammalian target of rapamycin (mTOR) and phosphorylated-mTOR expressions in the hippocampal CA1 region of rat with vascular dementia. Journal of Veterinary Science, 18(1), 11-16.

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Quantitative Analysis of mTOR and p-mTOR in Hippocampal CA1

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According to the method described in our previous studies [14 (link)15 (link)16 (link)19 (link)], immunohistochemistry for mTOR and p-mTOR was performed with rabbit anti-mTOR (1:100; Santa Cruz Biotechnology, USA) or rabbit anti-p-mTOR (ser2448) (1:100; Santa Cruz Biotechnology) as the primary antibody.
Eight sections at 120 µm intervals were selected per animal to quantitatively analyze mTOR and p-mTOR. Digital images of the hippocampal CA1 region were captured with an Axio Imager 2 microscope (Carl Zeiss) equipped with an Axiocam digital camera (Carl Zeiss) connected to a PC monitor.

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Comprehensive Protein Analysis Protocol

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Nuclear and Cytoplasmic Protein Extraction Kit (Beyotime Biotech, China); Lipofectmin 2000 (Invitrogen, USA); Entranster™-in vivo transfection reagent (Engreen Biosystem, China); pifithrin-α (Sigma, USA); fluvastatin (Novartis Pharma, Switzerland); 3-methyadenine (Sigma, USA); Bafilomycin A1 (Santa Cruz, CA, USA), chloroquine (MedChemExpress, USA), denosumab (Prolia, Amgen Inc., USA); mouse-anti-AMPKα1/2 (sc-74461, Santa Cruz, USA); rabbit-anti-pAMPKα1/2 (Thr 172) (sc-33524, Santa Cruz, USA); rabbit-anti-ACCα (sc-30212, Santa Cruz); rabbit-anti-ACCα (sc-271965, Santa Cruz); mouse-anti-PTEN (sc-7974, Santa Cruz); rabbit-anti-AKT (sc-8312, Santa Cruz); rabbit-anti-pAKT (Ser 473) (sc-33437, Santa Cruz); rabbit-anti-Histone H3 (sc-10809, Santa Cruz); mouse-anti-p53 (sc-126, Santa Cruz); rabbit-anti-LC3 (sc-28266, Santa Cruz); rabbit-anti-mTOR (sc-8319, Santa Cruz); rabbit-anti-mTOR (Ser 2448) (sc-101738, Santa Cruz); mouse-anti-β-Actin (sc-47778, Santa Cruz).

Yang Z., Su Z., DeWitt J.P., Xie L., Chen Y., Li X., Han L., Li D., Xia J., Zhang Y., Yang Y., Jin C., Zhang J., Li S., Li K., Zhang Z., Qu X., He Z., Chen Y., Shen Y., Ren M, & Yuan Z. (2017). Fluvastatin Prevents Lung Adenocarcinoma Bone Metastasis by Triggering Autophagy. EBioMedicine, 19, 49-59.

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