Anti nono

Anti-Nono (Cat# 11058-1-AP and 66361-1-Ig) was from Proteintech. Anti-Elk3 (Cat# NBP1-83960) was from Novus Biologicals. Anti-Tmem241 (Cat# 203644-T32) was from Sinobiological. Anti-Lineage cocktail (Cat# 88-7772-72), Anti-CD127 (A7R34), anti-Sca-1 (D7), anti-Flt3 (A2F10), anti-α₄β₇ (DATK32), anti-Id2 (ILCID2), anti-PLZF (Mags.21F7), anti-Eomes (Dan11mag), anti-NKp46 (29A1.4), anti-NK1.1 (PK136), anti-CD45 (30-F11), anti-CD25 (PC61.5), anti-Gata3 (TWAJ), anti-RORγt (AFKJS-9), anti-Bcl11b (1F8H9), anti-CD3 (17A2), anti-CD19 (1D3), anti-KLRG1 (2F1), anti-CD90 (HIS51), anti-IL-22 (IL22JOP), anti-Ki67 (SolA15), anti-CD45.2 (104), anti-BrdU (BU20A), anti-PD-1 (J43) and anti-CD45.1 (A20) were purchased from eBiosciences (San Diego, USA). Anti-c-Kit (2B8), anti-CD150 (TC15-12F12.2), anti-CD48 (HM48-1), and anti-CD49a (HMα1) were purchased from Biolegend (California, USA). Anti-β-actin (Cat# RM2001) was purchased from Beijing Ray Antibody Biotech. Paraformaldehyde (PFA) and 4’,6-diamidino-2-phenylindole (DAPI) were from Sigma. IL-22 ELISA kit was purchased from Neobioscience.

Pathogen-free C57BL/6 (10-week-old) mice were procured from Vital River Laboratory Animal Technology (Beijing, China). All animal care and use conformed to the Association for Research in Vision and Ophthalmology (ARVO) Statement for the Use of Animals in Ophthalmic and Vision Research, and the experimental protocols were approved by the Animal Care and Use Committee of Tianjin Medical University (ethics approval number: TJYY2019110117).
The interphotoreceptor retinoid-binding protein (IRBP) peptide 1–20 (aa 1–20 of IRBP) peptide was synthesized and purified by Sangon Biotech (Shanghai, China). Complete Freund’s adjuvant (CFA) and pertussis toxin were purchased from Sigma Aldrich (St Louis MO, USA). Mycobacterium tuberculosis H37RA was obtained from BD Biosciences (San Jose, CA, USA). The recombinant murine cytokines IL-23, IL-4, and GM-CSF were obtained from R&D Systems (Minneapolis, MN, USA). PE-conjugated, FITC-conjugated, or APC-conjugated antibodies against mouse CD4, IL-17, IFN-γ, Foxp3, CD40, and CD11c were purchased from BioLegend (San Diego, CA, USA). Anti-NonO was purchased from Proteintech (Chicago, IL, USA). The WP1066 was obtained from Calbiochem (San Diego, CA, USA), and dissolved in DMSO for storage and usage.