Log In Sign up
The largest database of trusted experimental protocols

Based mbp affinity chromatography

Manufactured by GE Healthcare
Visit Supplier

Based MBP affinity chromatography is a purification technique used to isolate and purify proteins that have an affinity for the maltose-binding protein (MBP) tag. It utilizes the specific interaction between MBP and its ligand to capture and separate the target protein from complex mixtures. The core function of this system is to provide an efficient and selective method for the purification of MBP-tagged recombinant proteins.

Automatically generated - may contain errors

Lab products found in correlation

Sigmaplot v12, by Grafiti LLC (2 mentions) Spectramax m2e, by Molecular Devices (2 mentions) Elisa plate, by Corning (2 mentions) 1 step ultra tmb elisa substrate, by Thermo Fisher Scientific (2 mentions)

2 protocols using based mbp affinity chromatography

1

AAVR Binding Assay Using ELISA

Check if the same lab product or an alternative is used in the 5 most similar protocols
Purification of the soluble AAVR was achieved through amylose-based MBP affinity chromatography (GE Healthcare). ELISA plates (Corning Costar) were coated overnight at 4°C with 50ul AAV2 virus-like particles (VLPs) at 2.5 μg/ml in 100 mM NaHCO3, pH 9.6. Plates were then washed 2X with TBST (0.05% Tween-20 in TBS) and blocked with 3% BSA in TBST for 1 hr at RT. Subsequent washing was followed by incubation with soluble AAVR or MBP control at the indicated concentrations for 2 hrs at RT. Anti-MBP-HRP (1:500, 1 hr incubation at RT) was used to detect rAAVR1-5 and MBP controls, requiring no secondary antibody. Samples were developed with 1-Step Ultra TMB-ELISA substrate per product instructions (Thermo Scientific, USA) and optical density assayed by microplate reader (Molecular Devices SpectraMax M2e) at 450 nm. Curve fitting was performed in SigmaPlot v12.5 (Systat Software, Inc., USA). All data presented is representative of at least three independent experiments.
Pillay S., Meyer N.L., Puschnik A.S., Davulcu O., Diep J., Ishikawa Y., Jae L.T., Wosen J.E., Nagamine C.M., Chapman M.S, & Carette J.E. (2016). An essential receptor for adeno-associated virus infection. Nature, 530(7588), 108-112.
+ Open protocol
+ Expand
2

AAVR Binding Assay Using ELISA

Check if the same lab product or an alternative is used in the 5 most similar protocols
Purification of the soluble AAVR was achieved through amylose-based MBP affinity chromatography (GE Healthcare). ELISA plates (Corning Costar) were coated overnight at 4°C with 50ul AAV2 virus-like particles (VLPs) at 2.5 μg/ml in 100 mM NaHCO3, pH 9.6. Plates were then washed 2X with TBST (0.05% Tween-20 in TBS) and blocked with 3% BSA in TBST for 1 hr at RT. Subsequent washing was followed by incubation with soluble AAVR or MBP control at the indicated concentrations for 2 hrs at RT. Anti-MBP-HRP (1:500, 1 hr incubation at RT) was used to detect rAAVR1-5 and MBP controls, requiring no secondary antibody. Samples were developed with 1-Step Ultra TMB-ELISA substrate per product instructions (Thermo Scientific, USA) and optical density assayed by microplate reader (Molecular Devices SpectraMax M2e) at 450 nm. Curve fitting was performed in SigmaPlot v12.5 (Systat Software, Inc., USA). All data presented is representative of at least three independent experiments.
Pillay S., Meyer N.L., Puschnik A.S., Davulcu O., Diep J., Ishikawa Y., Jae L.T., Wosen J.E., Nagamine C.M., Chapman M.S, & Carette J.E. (2016). An essential receptor for adeno-associated virus infection. Nature, 530(7588), 108-112.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!