Tecnai 20 200 kv feg electron microscope

Sorted DC subsets were pelleted by centrifugation and fixed in 0.1 M sodium cacodylate buffer, pH 7.4, containing 2.5% glutaraldehyde and 2% paraformaldehyde. Samples were post-fixed in 2% osmium tetroxide in 0.1 M cacodylate buffer with 0.15% potassium ferrocyanide, followed by dehydration to propylene oxide and embedding in epoxy resin. TEM images of ultrathin (80 nm) sections were acquired using a FEI Tecnai 20 200 KV FEG electron microscope.

After B/B stimulation, NIH3T3 cells are fixed in 2.5% glutaraldehyde and 2% paraformaldehyde in 0.1 M sodium cacodylate buffer pH 7.4 before post-fixation in 2% osmium tetroxide in 0.1 M cacodylate buffer with 0.15% potassium ferrocyanide. After rinsing in buffer the tissue was dehydrated through a series of graded ethanol to propylene oxide, infiltrated and embedded in epoxy resin and polymerized at 70°C overnight. Semi-thin sections (0.5 micron) are stained with toluidine blue for light microscope examination. Ultrathin sections (80 nm) are cut and imaged using the Tecnai TF20 TEM with an AMT XR41 camera. TEM images were acquired using A FEI Tecnai 20 200KV FEG Electron Microscope.