Dipotassium hydrogen phosphate k2hpo4

The dried root of A. membranaceus samples were obtained from Shanxi, Inner Mongolia, Gansu, and Heilongjiang provinces, respectively. They were screened for the consistency of shape, diameter, and length. The reagents used in the present work were of analytical grade. The methanol was purchased from Macklin Biochemical Co., Ltd. (Shanghai, China). Folin-Ciocalteu reagent, sodium carbonate (Na₂CO₃), aluminum chloride (AlCl₃), sodium nitrite (NaNO₂), sodium hydroxide (NaOH), potassium dihydrogen phosphate (KH₂PO₄), and dipotassium hydrogen phosphate (K₂HPO₄) were obtained from Sangon Biotech (Shanghai, China). Gallic acid, catechin, ascorbic acid, fluorescein sodium, trolox, 1,1-diphenyl-2-picryldydrazyl (DPPH), 3-(4,5-dimethylthiazol-2-yl)-5(3-carboxymethonyphenol)-2-(4-sulfophenyl)-2H-tetrazolium (MTS), glutamic acid, and 2,2′-azobis-amidinopropane (ABAP) were purchased from Sigma Chemical Co. (St. Louis, MO, USA). HPLC-grade methanol was obtained from Merck Co., Ltd. (Darmstadt, Germany). Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), and phosphate-buffered saline (PBS) for cell culture were purchased from Gibco Life Technologies (Grand Island, NY, USA). All the other chemicals were obtained from Tianjin Chemical Reagent Co. (Tianjin, China).

Folin–Ciocalteu reagent, gallic acid, daidzein, glycitin, L-ascorbic acid (ASA), 2,2’-azobis-amidinopropane (ABAP), Trolox and Fluorescein disodium salt were purchased from Sigma Aldrich (St. Louis, Mo, USA). Acetone, sodium hydroxide (NaOH), methanol, ethylenediamine tetra-acetic acid (EDTA), Sodium carbonate (Na₂CO₃), potassium dihydrogen phosphate (KH₂PO₄) and dipotassium hydrogen phosphate (K₂HPO₄) were purchased from Sangon Biotech Co. Ltd. (Shanghai, China). Acetonitrile and methanol (HPLC grade) were purchased from ANPEL Scientific Instrument Co. (Shanghai, China). Other reagents used were analytical grade.