Agmatine sulfate salt

Manufactured by Merck Group

Sourced in United States

Agmatine sulfate salt is a chemical compound used in various laboratory applications. It is a salt formed by the combination of the organic compound agmatine and sulfuric acid. Agmatine sulfate salt is commonly used as a reagent in biochemical and analytical procedures, but its specific core function is to serve as a chemical intermediate without further interpretation.

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9 protocols using agmatine sulfate salt

Quantitative Analysis of Biogenic Amines

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The reference standards of 1,3-diaminopropane, putrescine, cadaverine hydrochloride, spermidine hydrochloride, spermine, agmatine sulfate salt, N-acetylputrescine hydrochloride, N-acetylspermine trihydrochloride, N-acetylspermidine dihydrochloride, l-ornithine hydrochloride, lysine, l-arginine, S-adenosyl-l-methionine, γ-aminobutyric acid and 1, 6-diaminohexane (used as an internal standard) were all obtained from Sigma-Aldrich (St. Louis, MO, USA). HPLC grade methanol was purchased from Fisher Chemicals (Fair Lawn, NJ, USA). Heptafluorobutyric acid (HFBA) was obtained from Sigma-Aldrich. All the other reagents were of analytic grade. Redistilled and deionized water was used throughout the study.

Xu H., Liu R., He B., Bi C.W., Bi K, & Li Q. (2016). Polyamine Metabolites Profiling for Characterization of Lung and Liver Cancer Using an LC-Tandem MS Method with Multiple Statistical Data Mining Strategies: Discovering Potential Cancer Biomarkers in Human Plasma and Urine. Molecules, 21(8), 1040.

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Comprehensive Polyamine Metabolite Analysis

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The reference standards of putrescine, cadaverine hydrochloride, spermidine hydrochloride, spermine, agmatine sulfate salt, N-acetyl-putrescine hydrochloride, N-acetyl-spermine trihydrochloride, N-acetyl-spermidine dihydrochloride, L-ornithine hydrochloride, lysine, L-arginine, S-adenosyl-1-methionine, aminobutyric acid, deuterated histamine, heptafluorobutyric acid (HFBA), and methanol LC/MS grade were purchased from Sigma-Aldrich (St. Louis, MO, USA). Water for LCMS was purchased from Fisher Scientific (Fair Lawn, NJ, USA).

Garroni G., Balzano F., Cruciani S., Pala R., Coradduzza D., Azara E., Bellu E., Cossu M.L., Ginesu G.C., Carru C., Ventura C, & Maioli M. (2021). Adipose-Derived Stem Cell Features and MCF-7. Cells, 10(7), 1754.

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Heterologous Production of Fatty Acids

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Malonyl-CoA, NADPH, fatty acids, antibiotics, M17 medium and agmatine sulfate salt were purchased from Sigma-Aldrich. DNA polymerases, Gibson assembly kit, restriction endonucleases and T4 ligase were from NEB. Sodium [1-¹⁴C]acetate (specific activity, 58.6 mCi/mmol) was provided by Moravek. Ni-NTA resin and the DNA purification kits were from Qiagen, and the silver nitrate silica gel thin layer plates were from Analtech. All the other reagents were of the highest available quality. Oligonucleotide primers were synthesized by Integrated DNA Technologies and DNA sequencing was provided by ACGT, Inc.

Dong H, & Cronan J.E. (2022). Unsaturated fatty acid synthesis in Enterococcus faecalis requires a specific enoyl‐ACP reductase. Molecular Microbiology, 118(5), 541-551.

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Reagents for Pain and Inflammation Study

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Agmatine sulfate salt (Cat#a7127), histamine (Cat#y0001779), compound 48/80 (Cat#C2313), resiniferatoxin (RTX, Cat#R8756), chlorpheniramine maleate (Cat#C3025), chloroquine (CQ, Cat#C6628), naloxone hydrochloride (Cat#N3136), amiloride hydrochloride (Cat#1019701) were all obtained from Sigma-Aldrich (St. Louis, MO, United States). MC903 (Cat# 2700/10) was purchased from R&D systems (Minneapolis, MN, United States). Morphine hydrochloride was obtained from China Northeast Pharmaceutical Group Shenyang No. 1 Pharmaceutical Co., Ltd (Shenyang City, China). naloxone hydrochloride HC-030031 (Cat#2896), capsazepine (CPZ, Cat#0464) and APETx2 (Cat#4804) were purchased from Tocris (Minneapolis, MN, United States). ZnCl₂ was obtained from National Pharmaceutical Group pharmaceutical Co., Ltd (Beijing, China). RTX and HC-030031 were freshly dissolved in 10% DMSO. Other reagents were dissolved in saline. Information relating to timing and doses are indicated in the results section or figure legends.

Zhou G.K., Xu W.J., Lu Y., Zhou Y., Feng C.Z., Zhang J.T., Sun S.Y., Wang R.M., Liu T, & Wu B. (2023). Acid-sensing ion channel 3 is required for agmatine-induced histamine-independent itch in mice. Frontiers in Molecular Neuroscience, 16, 1086285.

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Lb. brevis 145 Acid Stress Response

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Cells of Lb. brevis 145 grown for 12 h (early stationary phase) in Lactobacilli MRS broth were inoculated into Lactobacilli MRS broth (pH 6.5) supplemented with or without the mixture of MSG, arginine, agmatine sulfate salt and tyrosine (1 g/L each; Sigma). However, tyrosine was partially dissolved in MRS broth (pH 6.5), and the insoluble tyrosine (solid form in the broth) was removed by membrane filtering (0.45 μm). The initial cell density after inoculation for acid challenge was 1.0 × 10⁸ CFU/mL and the cells were incubated at 37°C for 36 h statically. Samples were collected at the time point of 0, 3, 6, 9, 12, 18, 24, and 36 h. Subsequently, measurements of acids, amino acids, amines and gene expression were carried out as discussed below. Plate count method was used to assess the cell viability, and pH_in and pH_ex measurements were carried out as stated above.

Wu Q., Tun H.M., Law Y.S., Khafipour E, & Shah N.P. (2017). Common Distribution of gad Operon in Lactobacillus brevis and its GadA Contributes to Efficient GABA Synthesis toward Cytosolic Near-Neutral pH. Frontiers in Microbiology, 8, 206.

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Optimized Biomaterial Characterization Protocol

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PA, tris(hydroxymethyl)-aminomethane (Tris), NaCl, Alt sodium 3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC.HC1), N-hydroxysuccinimide, agmatine sulfate salt, 4-(4,6-Dimethoxy-l,3,5-triazin-2-yl)-4-methylmorpholinium chloride, PEI, HA, Hoechst 33342, 5-bromo-4-chloro-3-indoxylphosphate (BCIP), Nitro Blue tetrazolium (NBT), Alizarin red S, L-ascorbic acid, p-nitrophenol phosphate, β-glycerophosphate, dexamethasone were purchased from Sigma Aldrich (St.Louis, MO). 20S-OHC was obtained from R&D System Inc. (Minneapolis, MN). Cholesterol fluorometric assay kit was obtained from Cayman Chemical (Ann Arbor, MA). 5-FAM cadaverine and LysoTracker blue were purchased from AAT Bioquest Inc. (Sunnyvale, CA). PLGA (Poly-Lactic-Glycolic-Acid), cell culture media Dulbecco’s modified Eagle’s medium (DMEM), penicillin, streptomycin, Calcein AM, EthD-1, Quant-iT™ PicoGreen™ dsDNA Assay Kit, Trizol reagents and cDNA transcription kit were purchased from Life Technologies (Grand Island, NY).

Zhang X., Fan J., Lee C.S., Kim S., Chen C., Aghaloo T, & Lee M. (2020). Apatite-binding nanoparticulate agonist of hedgehog signaling for bone repair. Advanced functional materials, 30(12), 1909218.

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Ethanol Exposure and Agmatine Intervention in Rodents

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190 proof ethyl alcohol (mice: Warner Graham CO., Cockeysville, MD, rats: LSUHSC-New Orleans General Stores) was used to expose rodents to ethanol vapors and to prepare solutions (15% v/v for mice and 10% w/v for rats) to drink. Pyrazole, sucrose, and agmatine sulfate salt were obtained from Sigma-Aldrich (St. Louis, MO). The doses of agmatine were selected based on previous publications (Aricioglu & Regunathan, 2005 (link); Utkan, Gocmez, Regunathan, & Aricioglu, 2012 (link)).

Lopez M.F., Davis E.C., Cucinello-Ragland J.A., Regunathan S., Edwards S, & Becker H.C. (2023). Agmatine Reduces Alcohol Drinking and Produces Antinociceptive Effects in Rodent Models of Alcohol Use Disorder. Alcohol (Fayetteville, N.Y.), 109, 23-33.

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Biosynthesis of Fatty Acids

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Malonyl‐CoA, NADPH, fatty acids, antibiotics, M17 medium, and agmatine sulfate salt were purchased from Sigma‐Aldrich. DNA polymerases, Gibson assembly kit, restriction endonucleases and T4 ligase were from NEB. Sodium [1‐¹⁴C]acetate (specific activity, 58.6 mCi/mmol) was provided by Moravek. Ni‐NTA resin and the DNA purification kits were from Qiagen, and the silver nitrate silica gel thin layer plates were from Analtech. All the other reagents were of the highest available quality. Oligonucleotide primers were synthesized by Integrated DNA Technologies and DNA sequencing was provided by ACGT, Inc.

Dong H, & Cronan J.E. (2022). Unsaturated fatty acid synthesis in Enterococcus faecalis requires a specific enoyl‐ACP reductase. Molecular Microbiology, 118(5), 541-551.

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Quantitative Analysis of Polyamines

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The reference standards -1,3-diaminopropane, putrescine, cadaverine hydrochloride, spermidine hydrochloride, spermine, agmatine sulfate salt, N-acetylputrescine hydrochloride, N-acetylspermine trihydrochloride, Nacetylspermidine dihydrochloride, and 1,6-diaminohexane (used as an internal standard [IS]) -were all obtained from Sigma-Aldrich (St. Louis, MO). N 1 , N 12 -diacetylspermine n-hydrochloride and N 1 , N 8 -diacetylspermidine were obtained from Wako (Osaka, Japan). Methanol of high-performance liquid chromatography (HPLC) grade was purchased from Fisher Chemicals (Fair Lawn, NJ). Perfluoropentanoic acid was obtained from Sigma-Aldrich (St. Louis, MO).
Raw materials of Mylabris, Ginseng Radix et Rhizoma, Astragali Radix, and Acanthopanacis Senticosi Radix et Rhizoma Seu Caulis were purchased from Tianyitang Chinese Drugstore (Shenyang, China). These drugs were identified by a Professor of Pharmacognosy in Shenyang Pharmaceutical University (China). The Aidi injection was prepared according to the Ministerial Standards of Chinese Medicine. All other reagents were of analytical grade. Redistilled and deionized water was used throughout the study.

Li Q., Liu R., Zhu L., Yu C., Shuai Y., Sun L., & Bi K. (2021). Quantitative evaluation of the compatibility effects of aidi injection on the treatment of hepatocellular carcinoma using targeted metabolomics: A new strategy on the mechanism study of an anticancer compound in traditional chinese medicine. World Journal of Traditional Chinese Medicine, 7(1), 111-111.

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