Mouse osteoprotegerin elisa kit

Cell culture supernatants and serum were analyzed with a Mouse RANKL ELISA Kit (Abcam) and Mouse Osteoprotegerin ELISA Kit (Abcam). All experiments were performed according to the manufacturer's protocols.

To determine the concentrations of bone turnover markers (procollagen type I N-terminal propeptide (PINP); OPG; C-terminal telopeptide (CTX); RANKL), blood was harvested at an age of 12 or 36 weeks and 8 weeks after OVX or sham operation in microvettes (Sarstedt AG & Co., Nümbrecht, Germany), centrifuged at 13,000 × g for 7 min for serum collection and stored at −80°C until further use. Bone turnover markers were determined using RatLaps™ CTX-I EIA, Rat/Mouse PINP EIA (both from Immunodiagnostic Systems GmbH, Frankfurt am Main, Germany), mouse RANKL ELISA Kit (TNFSF11), and mouse Osteoprotegerin ELISA Kit (TNFRSF11B) (both from Abcam, Cambridge, United Kingdom) according to manufacturers’ protocols. Using a customized mouse Multiplex Cytokine Kit (ProcartaPlex, eBiscience, Frankfurt, Germany), the CXCL-1, CXCL-10, IL-6, monocyte chemoattractant protein-1 (MCP-1), macrophage colony-stimulating factor (M-CSF), and vascular endothelial growth factor (VEGF) concentrations were determined in the OVX and Sham mice.