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Mouse anti lamp2 sc 18822

Manufactured by Santa Cruz Biotechnology
Sourced in Germany

Mouse anti-LAMP2 (sc-18822) is a primary antibody that recognizes the LAMP2 (Lysosome-associated membrane glycoprotein 2) protein. LAMP2 is a type I transmembrane glycoprotein that is predominantly localized in the lysosomal membrane and is involved in the regulation of lysosomal function.

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3 protocols using mouse anti lamp2 sc 18822

1

Immunodetection of Cellular Organelles

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Mouse anti-Flag (F3165), rabbit anti-STX17 (HPA001204, Sigma, Jacksonville Registrant State, USA), rabbit anti-LC3 (L7543), rabbit anti-ERGIC53 (MFCD07370310) and mouse anti-β-tubulin (T8328) antibodies were purchased from Sigma-Aldrich (Jacksonville, FL, USA). Mouse anti-LAMP2 (sc-18822) and mouse anti-Tom20(sc-17764) antibodies were purchased from Santa Cruz. Rabbit anti-p62 (PM045, MBL) antibody was purchased from MEDICAL & BIOLOGICAL LABORATORIES CO. (MBL) (Woburn, MA, USA). Mouse anti-p62 (H00008878-M01) antibody was purchased from Novus. Mouse anti-GM130 (610822) was purchased from BD Biosciences (San Jose, CA, USA). Mouse anti- conjugated ubiquitin FK2 antibody (PW8810) was purchased from Biomol Res. The antibodies are diluted according to the instructions of each company.
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2

Protein Detection and Autophagy Assays

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Rabbit anti-LC3B (L7543) and mouse anti-betaActin (A5441) were from Sigma-Aldrich (Saint-Quentin-Fallavier, France). Guinea pig anti-p62 (GP62-C) was from PROGEN Biotechnik GmbH (Heidelberg, Germany). Rabbit anti-mTOR (2972S), rabbit anti-p-mTOR (2971S), rabbit anti-p-Akt (4058S), rabbit anti-Akt (9272S) and rabbit anti-PARP (9542) were from Cell Signaling (Danvers, MA, USA). Rabbit-anti-p-PERK (sc-32577), rabbit anti-GRP78 (H-129), mouse anti-Lamp1 (H4A3) and mouse-anti-Lamp2 (sc-18822) were from Santa Cruz Biotechnology (Heidelberg, Germany).
ML-9 (105637) was from Enzo Life Sciences (Villeurbanne, France). Acridine orange (A-3568) was from Life Technologies (Grand Island, NY, USA). Bafilomycin A1 (1334) was from Tocris (Bristol, UK). Chloroquine (C6628), Wortmannin (W1628) and trypan blue solution (T8154) were from Sigma-Aldrich.
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3

Melanogenesis Pathway Protein Analysis

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Mouse Anti-TYR (T311, sc-20035, Santa Cruz Biotechnology) antibody was used for Western blot and rabbit anti-TYR antiserum (PEP7, a gift from Dr V. J. Hearing, NCI, National Institute of Health) antibody was used for IF. Rabbit anti-PMEL17 (PEP13) antibody was a gift from Dr V. J. Hearing (NCI, National Institute of Health). Mouse anti-cathepsin D (sc-377299), mouse anti-TYRP-1 (sc166857), goat anti-TRP2/DCT (sc-74439), mouse GAP-DH (sc-81545), mouse anti-Rab38 (sc-390176), rabbit anti-flotillin-1 (sc-25506) and mouse anti-LAMP-2 (sc-18822) antibodies were from Santa Cruz Biotechnology. Rabbit anti-NPC1 (ab108921), mouse anti-MITF (ab80651), rabbit anti-Calnexin (ab22595) and rabbit anti-tubulin (ab18251) antibodies were purchased from Abcam. Mouse anti-HMB-45 (M06340-1) antibody was from Agilent. LysoTracker Green (DND-26 #8783) was from Cell Signaling Technology. Propidium iodide, cycloheximide and 3,4 dihydroxy-L-phenylalanine (L-DOPA, D9628), 2-hydroxypropyl-β-cyclodextrin (H107) were from Sigma-Aldrich. MG132 (sc-201260), leupeptin hemisulfate (sc-295358) and pepstatin A (sc-45036) were from Santa Cruz Biotechnology.
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