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Stannous 2 octoate sn oct 2

Manufactured by Merck Group
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Stannous (II) octoate (Sn(Oct)2) is a metal-organic compound containing tin. It is a colorless to pale yellow liquid with a characteristic odor. Stannous (II) octoate is commonly used as a catalyst or initiator in various chemical reactions and processes.

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5 protocols using stannous 2 octoate sn oct 2

1

Stem Cell Culture and Differentiation

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Polyethylene oxide monomethyl ether (PEO45, PEO148, PEO230, Sigma Aldrich), poly(ε-caprolactone) (PCL394, average Mn 45,000 g/mol, Sigma Aldrich, St. Louis, MO, USA), ε-caprolactone (ε-CL, purity: 97%, Sigma Aldrich, St. Louis, MO, USA) (see Figure 1), stannous (II) octoate (SnOct2, purity: 95%, Sigma Aldrich, St. Louis, MO, USA), tetrahydrofuran (THF, purity ≥99.5%, Merck, Darmstadt, Germany), n-hexane (purity ≥98.5%, Merck, Darmstadt, Germany), and dichloromethane (DCM, purity ≥99.8%, Merck, Darmstadt, Germany), were used as received. Culture medium (NeuroCult and NeuroCult NSA proliferation medium-rat, STEMCELL Technologies, Vancouver, BC, Canada) were used to culture stem cells. The following primary antibodies were used for immunofluorescence assays: (i) glial fibrillary acidic protein (GFAP, astrocyte marker), polyclonal raised in rabbit (1:750 dilution, Sigma, St. Louis, MO, USA); (ii) β-III tubulin (neuronal marker), monoclonal (1:750 dilution, Sigma, St. Louis, MO, USA); 1 mg/mL DAPI solution for nucleus staining (4′,6-diamidino-2-phenylindole dihydrochloride, Molecular Probes, Thermo Fisher Scientific, Waltham, MA, USA). Secondary antibodies conjugated with Alexa Fluor 488 or 594 (1:500 dilution, Invitrogen, Carlsbad, CA, USA) were also used.
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2

Rapamycin-Loaded Polymeric Nanocarriers

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Rapamycin was purchased from LC Laboratories (Woburn, MA). Poly(amidoamine) (PAMAM; fourth generation) dendrimer was purchased from NanoSynthons, LLC (Mt. Pleasant, MI, USA). Dimethyl sulfoxide (DMSO), valerolactone (VL), lactide (LA), glycolide (GA), Tween 80, paraformaldehyde, and stannous (II) octoate (Sn(Oct)2) were purchased from Sigma-Aldrich (St. Louis, MO). HOOC–PEG–OCH3 (Mn = 5 kDa) was acquired from JenKem Technology (Allen, TX, USA). Kolliphor P407 (a poly-(ethylene oxide)–poly(propylene oxide)–poly(ethylene oxide) tri-block copolymer) was kindly provided by BASF Corporation (Tarrytown, NY) and was used to prepare the pluronic gel (30 wt % of polymers in DI water). OH–PEG–OH (Mn = 1 kDa) and all other reagents were purchased from ThermoFisher Scientific (Fitchburg, WI, USA) unless otherwise specified.
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3

Platelet Membrane Isolation and Characterization

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Human platelets and platelet membranes were purchased from Zen-Bio Inc (Research Triangle Park, NC). RNAlater solution, TRIzol, SuperScript IV VILO Master Mix, and SYBR Green PCR Mastermix was purchased from Thermo Fisher Scientific (Waltham, MA). Poly(amidoamine) (PAMAM; 4th generation dendrimer), Evans Blue, dimethyl sulfoxide (DMSO), valerolactone (VL), and stannous (II) octoate (Sn(Oct)2) were purchased from Sigma-Aldrich (St. Louis, MO). Rapamycin was purchased from LC Laboratories (Woburn, MA). JQ1 was purchased from ApexBio (Houston, TX). Cy5 dye was obtained from Lumiprobe Co. (Hallandale Beach, FL). Reagents not otherwise specified were purchased from Thermo Fisher Scientific (Fitchburg, WI).
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4

Synthesis and Characterization of Multifunctional Poly(ethylene glycol) Conjugates

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Boltron® H40 (a hyperbranched polyester with 64 hydroxyl terminal groups; Mn: 2833 Da) was kindly provided by Perstorp Polyols Inc., USA, and purified by fractional precipitation in acetone and tetrahydrofuran (THF). β-Benzyl L-aspartate N-carboxyanhydride (BLA-NCA) was prepared as previously reported by our lab [49 (link)]. The heterobifunctional poly(ethylene glycol) (PEG) derivatives, methoxy–PEG–NH2 (CH3O-PEG–NH2, Mn = 5 kDa) and maleimide–PEG–NH2 (Mal–PEG–NH2, Mn = 5 kDa), were purchased from JenKem Technology (Allen, TX, USA). Cy5 dye was obtained from Lumiprobe Corporation (Hallandale Beach, FL, USA). GE11 peptide (YHWYGYTPQNVIGGGGC) was synthesized by Tufts University Core Facility (Boston, MA, USA). GFP-siRNA-Cy5.5, GFP-siRNA, dimethyl sulfoxide (DMSO), 2-carboxybenzylaldehyde, 2-aminoethyl disulfide, 4-imidazolecarboxylic acid, and stannous (II) octoate (Sn(Oct)2) were purchased from Sigma–Aldrich (St. Louis, MO, USA). 4-Dimethylamino pyridine (DMAP) and 1,3-dicyclohexylcarbodiimide (DCC) were purchased from ACROS and used without further purification. Other reagents, including RNAiMAX, were purchased from Thermo Fisher Scientific (Fitchburg, WI, USA) and used as received unless otherwise stated.
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5

Dendrimer-Mediated Drug Delivery System

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Poly(amidoamine) (1,4-diaminobutane; G4) dendrimer was purchased from NanoSynthons LLC (Mt. Pleasant, MI, USA). Dimethyl sulfoxide (DMSO), velarolactone (VL), and stannous (II) octoate (Sn(Oct)2) were purchased from Sigma–Aldrich (St. Louis, MO, USA). The heterobifunctional poly(ethylene glycol) (PEG) derivatives, methoxy–PEG–OH (mPEG–OH, Mn=5 kDa) and OH–PEG–N–hydroxylsuccinimide (HO–PEG–NHS, Mn=5 kDa) were purchased from JenKem Technology (Allen, TX, USA). 4-Dimethylamino pyridine (DMAP) and 1,3-dicyclohexylcarbodiimide (DCC) were purchased from ACROS and used without further purification. KE108 was purchased from Bachem Americas, Inc. (Torrance, CA, USA). Cy5 dye was obtained from Lumiprobe Corporation (Hallandale Beach, FL, USA). Other reagents were purchased from Thermo Fisher Scientific (Fitchburg, WI, USA) and used as received unless otherwise stated.
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