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Anti mcd8

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Anti-mCD8 is a monoclonal antibody that binds to the mouse CD8 antigen. It is a useful tool for the identification and isolation of CD8+ T cells in mouse models.

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Lab products found in correlation

Gemcitabine, by Merck Group (2 mentions) Ly364947, by Merck Group (2 mentions) Anti pd l1, by BioLegend (2 mentions)

Spelling variants of this product

Anti-mCD8 clone 2.43 (2 citations)

2 protocols using anti mcd8

1

Pancreatic Tumor Treatment Regimen

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In all animals, drug administration started after ultrasound confirmed a pancreatic tumor measuring ≥4–6 mm in largest linear dimension or 200–250 mm3 in total volume for KP16 and ≥5–10 mm or 250–500 mm3 for KPC tumors. Mice were randomized to respective treatment arms. LY364947 (Sigma-Aldrich) was diluted in 200 μL PBS and injected intraperitoneally (i.p.) at 1 mg/kg body weight as described previously (22 (link)). Gemcitabine (Sigma-Aldrich, catalog no. G6423) was diluted in 100 μL normal saline and injected i.p. every 4 days at 50 mg/kg. Anti-PD-L1 (BioLegend, catalog no 124329) was administered twice a week at 150 μg via i.p. injection, for CD8 T-cell depletion, two doses of 100 μg anti-mCD8 (Bioxcell, catalog no BE0061) or rat isotype control IgG1 (Bioxell, catalog no BE0090) per mouse on day 1 and 5 were administered.
Li D., Schaub N., Guerin T.M., Bapiro T.E., Richards F.M., Chen V., Talsania K., Kumar P., Gilbert D.J., Schlomer J.J., Kim S.J., Sorber R., Teper Y., Bautista W., Palena C., Ock C.Y., Jodrell D.I., Pate N., Mehta M., Zhao Y., Kozlov S, & Rudloff U. (2021). T Cell–Mediated Antitumor Immunity Cooperatively Induced By TGFβR1 Antagonism and Gemcitabine Counteracts Reformation of the Stromal Barrier in Pancreatic Cancer. Molecular Cancer Therapeutics, 20(10), 1926-1940.
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2

Evaluation of Combination Therapies in Pancreatic Cancer

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In all animals, drug administration started after ultrasound confirmed a pancreatic tumor measuring ≥4–6mm in largest linear dimension or 200–250mm3 in total volume for KP16 and ≥5–10mm or 250–500mm3 for KPC tumors. Mice were randomized to respective treatment arms. LY364947 (Sigma-Aldrich, St. Louis, MO) was diluted in 200μl PBS and injected intraperitoneally (IP) at 1mg/kg body weight as previously described (22 (link)). Gemcitabine (Sigma-Aldrich, Cat. #G6423) was diluted in 100μl normal saline and injected IP every 4 days at 50mg/kg. Anti-PD-L1 (Biolegend, Cat. #124329) was administered twice a week at 150μg via IP injection, for CD8 T cell depletion, two doses of 100μg anti-mCD8 (Bioxcell, Cat. #BE0061) or rat isotype control IgG1 (Bioxell, Cat. #BE0090) per mouse on day 1 and 5 were administered.
Li D., Schaub N., Guerin T.M., Bapiro T.E., Richards F.M., Chen V., Talsania K., Kumar P., Gilbert D.J., Schlomer J.J., Kim S.J., Sorber R., Teper Y., Bautista W., Palena C., Ock C.Y., Jodrell D.I., Pate N., Mehta M., Zhao Y., Kozlov S, & Rudloff U. (2021). T Cell–Mediated Antitumor Immunity Cooperatively Induced By TGFβR1 Antagonism and Gemcitabine Counteracts Reformation of the Stromal Barrier in Pancreatic Cancer. Molecular Cancer Therapeutics, 20(10), 1926-1940.
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