Ab8402

Manufactured by Abcam

Ab8402 is a primary antibody produced in rabbit. It is designed for use in immunohistochemistry applications.

Automatically generated - may contain errors

Lab products found in correlation

Ab10983, by Abcam (1 mentions) A1978, by Merck Group (1 mentions) Ripa lysis and extraction buffer, by Thermo Fisher Scientific (1 mentions) Chemidoc xr, by Bio-Rad (1 mentions) Ecl western blotting substrate, by Thermo Fisher Scientific (1 mentions) Quantity one software, by Bio-Rad (1 mentions) Ab106410, by Abcam (1 mentions) Gtx100118, by GeneTex (1 mentions) Gtx10983, by GeneTex (1 mentions) Ab77115, by Abcam (1 mentions) 0.45 um pvdf membrane, by Merck Group (1 mentions) Protease inhibitor cocktail, by Roche (1 mentions)

4 protocols using ab8402

Western Blot Analysis of Adipose Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

Fat tissues were lysed in a RIPA Lysis and Extraction Buffer (Thermo, USA) supplemented with protease and phosphatase inhibitors (genDEPOT, USA). Proteins were separated by SDS-PAGE and transferred to a transfer membrane. Membranes were washed 2 × 10 min with TBST (0.247 M Tris, 27 mM potassium chloride, 1.37 M sodium chloride, 0.5% Tween-20) and blocked with 5% skim milk based on TBST for 1 h at room temperature. After 10 min washing, the membranes were incubated with primary antibodies against UCP1 (ab10983, abcam, 1:1000), Cidea (ab8402, abcam, 1:1000), and β-Actin (A1978, Sigma, 1:10000) at 4°C overnight. Membranes were washed for 1 h, incubated with secondary antibodies (anti-rabbit 1:5,000 and anti-mouse 1:5,000) for 1 h 30 min at room temperature and washed for 6 times for 10 min each time. Each membrane was then placed in a detection solution (Bio-RAD, USA), followed by incubation for 2 min at room temperature and detection by ChemiDoc XRS+ (Bio-RAD, USA).

Kim J.K., Go H.S., Kim S.P., Kim I.Y., Lee Y.H., Oh S.H., Lee H, & Seong J.K. (2022). Exercise-induced beige adipogenesis of iWAT in Cidea reporter mice. BMB Reports, 55(4), 187-191.

+ Open protocol

+ Expand

Western Blot Protein Analysis Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Total protein was extracted from frozen tissues or cultured cells. Quantified protein was separated by SDS-PAGE, transferred onto polyvinylidene fluoride membranes, and immunoblotted with primary antibodies and secondary antibodies sequentially. Membranes were visualized by using ECL Western Blotting Substrate (Thermo Fisher Scientific). Band intensity was quantified by using Quantity One software (Bio-Rad) and normalized to total protein or α-tubulin.
The following primary antibodies were used: anti-insulin receptor (IR) (3025S), antiphospho-IR (3024S), anti-Akt (9272S), antiphospho-Akt (Ser473, 9271S), anti-Met (3127), antiphospho-Met (3126S), anti-acetyl-CoA carboxylase (ACC) (3662), and anti-stearoyl-CoA desaturase 1 (SCD-1) (2438) from Cell Signaling Technology; anti-α-tubulin (T6199) and anti-FLAG (F3165) from Sigma-Aldrich; anti-cell death–inducing DNA fragmentation factor-α–like effector a (Cidea) (ab8402) from Abcam; anti-lymphocyte antigen 6 complex, locus D (Ly6d) (17361–1-AP) from Proteintech; and anti-MR (sc-11412) and anti-fatty acid synthase (FAS) (sc-48357) from Santa Cruz Biotechnology.

Zhang Y.Y., Li C., Yao G.F., Du L.J., Liu Y., Zheng X.J., Yan S., Sun J.Y., Liu Y., Liu M.Z., Zhang X., Wei G., Tong W., Chen X., Wu Y., Sun S., Liu S., Ding Q., Yu Y., Yin H, & Duan S.Z. (2017). Deletion of Macrophage Mineralocorticoid Receptor Protects Hepatic Steatosis and Insulin Resistance Through ERα/HGF/Met Pathway. Diabetes, 66(6), 1535-1547.

+ Open protocol

+ Expand

Western Blot Analysis of Thermogenic Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

Samples prepared with Laemmli sample buffer and were separated by 10% SDS-PAGE gel and transferred to PVDF membrane. The membrane was blocked with 10% skim milk in PBST and incubated at 4 °C overnight with rabbit anti-4-HNE antibody (1:1000; cat. no. PAB1295, Abnova) and then with secondary antibodies with HRP(1:10000; cat. no. GTX26721, GeneTex). Immunoblots for Ucp1, Prdm16, Pgc1α, Dio2, Cidea, Hsp70, and Gapdh were performed using rabbit polyclonal anti-Ucp1 antibody (1:1000, cat. no. GTX10983, GeneTex), rabbit polyclonal anti-Prdm16 (1:1000, cat. no. ab106410, Abcam), rabbit polyclonal anti-Pgc1α antibody (1:1000, cat. no. NBP1-04676PCP, Novus), rabbit polyclonal anti-Dio2 antibody (1:1000, cat. no. GTX81072, GeneTex), rabbit polycloncal anti-Cidea (1:1000, cat. no. ab8402, Abcam), rabbit monoclonocal anti-Hsp70antibody (1:4000. cat. no.ab45133, Abcam), and rabbit polyclononal anti-Gapdh antibody (1: 5000,GTX100118, GeneTex).

Chang Y.C., Lee H.L., Yang W., Hsieh M.L., Liu C.C., Lee T.Y., Huang J.Y., Nong J.Y., Li F.A., Chuang H.L., Ding Z.Z., Su W.L., Chueh L.Y., Tsai Y.T., Chen C.H., Mochly-Rosen D, & Chuang L.M. (2023). A common East-Asian ALDH2 mutation causes metabolic disorders and the therapeutic effect of ALDH2 activators. Nature Communications, 14, 5971.

+ Open protocol

+ Expand

Western Blot Analysis of Cidea and Fsp27

Check if the same lab product or an alternative is used in the 5 most similar protocols

Liver tissues and AML12 cell protein lysates were prepared using RIPA buffer containing complete EDTA-free protease inhibitor cocktail (Roche). A total of 30–50 ng of protein was subjected to 12% SDS-PAGE and transferred to a 0.45 um PVDF membrane (Millipore, Bedford, MA, USA). The membranes were probed with antibodies to Cidea (1:1000; ab8402, abcam) and Fsp27 (1:1000; ab77115, abcam) detected using chemiluminescence reagents.

He Q., Diao Y., Zhao T., Hou B., Ngokana L.D., Liang H., Nie J., Tan P., Huang H., Li Y., Qi L., Zhao Y., Liu Y., Gao X, & Zhou L. (2018). SREBP1c mediates the effect of acetaldehyde on Cidea expression in Alcoholic fatty liver Mice. Scientific Reports, 8, 1200.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!