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Biomax mr

Manufactured by Carestream
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Sourced in United States

The BioMax MR is a film-based imaging system designed for medical radiography applications. It provides consistent image quality and reliable performance for healthcare professionals. The core function of the BioMax MR is to capture and process radiographic images for diagnostic purposes.

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Lab products found in correlation

Ecl plus, by Thermo Fisher Scientific (3 mentions) Oct compound, by Sakura Finetek (1 mentions) Sam2 biotin capture membrane, by Promega (1 mentions) Recombinant human cdk1 cyclin b, by New England Biolabs (1 mentions) Nebuffer for protein kinase, by New England Biolabs (1 mentions) Protease and phosphatase inhibitors cocktail, by Merck Group (1 mentions) Bradford assay, by Merck Group (1 mentions) Semi dry transfer system, by Bio-Rad (1 mentions)

Close spelling variants of this product

Biomax MR film

3 protocols using biomax mr

1

In Situ Hybridization of Cebelin in Mouse Brain

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Mouse brain at P56 was frozen in O.C.T. compound (Sakura Finetek), and sections were cut at 10 μm. A 35S-labeled sense or antisense RNA probe was transcribed from Cebelin cDNA clone. The signals were visualized by autoradiography using BioMax MR (Carestream) as described (Yazaki et al., 1994 (link)). The sections of mouse brain were counterstained with cresyl-violet (Nissl staining).
Miwa H, & Itoh N. (2018). Unknown genes, Cebelin and Cebelin-like, predominantly expressed in mouse brain. Heliyon, 4(9), e00773.
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2

Cdk1-Mediated Peptide Phosphorylation

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Synthetic peptides (50 μM final concentration) were incubated with 40 units of recombinant human Cdk1-cyclin B (New England Biolabs) in 1× NEBuffer for Protein Kinases (New England Biolabs) with 100 μM cold ATP and 5 μCi γ-[32P]ATP in a reaction volume of 20 μl. Reactions were incubated at 30°C for 30 min, then terminated by the addition of 10 μl 7.5 M guanidine-hydrochloride. 1.2 μl of each reaction was spotted onto an avidin-coated membrane (SAM2 biotin capture membrane, Promega). After air-drying, the membrane was rinsed once with 2 M NaCl, then incubated for 3 × 2 min in 2 M NaCl, 4 × 2 min in 2 M NaCl + 1% H3PO4, rinsed twice in distilled water, and air-dried at room temperature for 1 hr. The dried membrane was exposed to an autoradiography film (Carestream BioMax MR) overnight at −80°C.
Novak Z.A., Wainman A., Gartenmann L, & Raff J.W. (2016). Cdk1 Phosphorylates Drosophila Sas-4 to Recruit Polo to Daughter Centrioles and Convert Them to Centrosomes. Developmental Cell, 37(6), 545-557.
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3

Quantitative Western Blot Analysis

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Total protein extracts were obtained from cells using sterile cell scraper and NETN buffer (150 mM NaCl, 1 mM EDTA, 20 mM Tris-HCl pH 8.0, 0.5% Nonidet P-40) and 1% of protease and phosphatase inhibitors cocktail (Sigma-Aldrich). After extraction, protein concentration was quantified by Bradford assay (Sigma-Aldrich), according to the manufacturer recommendations. SDS-PAGE was performed with 30–50 µg of protein, followed by transference to a polyvinylidene fluoride membrane (PVDF; Bio-Rad, Hercules, CA, USA) using the semi-dry transfer system (Bio-Rad) for 90 minutes. Immunoblotting was performed by standard methods. Primary antibodies against human PLCδ4 (Santa Cruz Biotech; 1:200) and α-tubulin (Sigma-Aldrich; 1:2000) were used. Protein bands were revealed by chemiluminescence using ECL Plus (Thermo Scientific) and BioMax® MR (Carestream Dental, Atlanta, GA, USA) films. The films were scanned and quantitative analyses were performed using ImageJ software [34 (link)]. Relative protein expression was calculated using α-tubulin as a loading control. At least three assays were performed.
Kunrath-Lima M., de Miranda M.C., da Fonseca Ferreira A., Faraco C.C., de Melo M.I., Goes A.M., Rodrigues M.A., Faria J.A, & Gomes D.A. (2018). Phospholipase C delta 4 (PLCδ4) is a nuclear protein involved in cell proliferation and senescence in mesenchymal stromal stem cells. Cellular signalling, 49, 59-67.
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