D 10 hemoglobin testing program

Baseline information, including age, sex, comorbidities, smoking status, alcohol intake, medications, body height, weight, and blood pressure, was collected from medical records. Laboratory assessments consisted of fasting blood glucose, liver and renal function, uric acid, total cholesterol, triglycerides, low-density lipoprotein cholesterol (LDL-C), and high-density lipoprotein cholesterol (HDL-C), which were examined by a HITACHI (Tokyo, Japan) 7180 Automatic Analyser using 8-h overnight fasting blood samples. HbA1c was measured by high-performance liquid chromatography (HPLC) with a D-10 hemoglobin testing program (Bio-Rad). White blood cell measurement was performed with an automated hematology Analyser XE-1200 (Sysmex, Kobe, Japan). The MHR ratio was calculated by dividing the monocyte count by HDL-C.

Body mass index (BMI) was calculated (kg/m²). Systolic blood pressure (SBP) and diastolic blood pressure (DBP) taken three times using a sphygmomanometer and then averaged. Capillary glucose concentrations were measured with Lifescan Surestep blood glucose meter. Plasma glucose levels were measured using the glucose oxidase method. HbA1c was measured by high performance liquid chromatography (HPLC) with D-10 hemoglobin Testing Program (Bio-Rad). The serum insulin assay used magnetic beads-based enzymatic spectrofluorometric immunoassay with automatic enzyme immunoassay apparatus (AIA360, TOSOH). Serum glucose concentrations, total cholesterol (TC), triglyceride (TG), high density lipoprotein cholesterol (HDLC), and low density lipoprotein cholesterol (LDLC) were measured with Hitachi Model 7600 Series Automatic Analyzer.

Body mass index (BMI) was calculated (kg/m²). SBP and DBP were taken three times using a sphygmomanometer and then were averaged. Capillary glucose concentrations were measured with Lifescan Surestep blood glucose meter. Plasma glucose levels were measured using the glucose oxidase method. HbA1c was measured by high performance liquid chromatography(HPLC) with D-10 hemoglobin Testing Program (Bio-Rad). The serum insulin assay used magnetic beads-based enzymatic spectrofluorometric immunoassay with automatic enzyme immunoassay apparatus (AIA360, TOSOH). Serum glucose concentrations, total cholesterol (TC), triglyceride (TG), high density lipoprotein cholesterol (HDLC), low density lipoprotein cholesterol (LDLC), and serum creatinine(Scr) were measured with Hitachi Model 7600 Series Automatic Analyzer. Glomerular filtration rate(GFR) was estimated by using the reexpressed 4-variable Modification of Diet in Renal Disease (MDRD) Study equation (eGFR = 175 × (standardized Scr) ^–1.154 × age^–0.203 × 0.742 [if female]) [22 (link)].