The human RCC cell lines (OS-RC-2, Caki-2, Caki-1, A498, 786-O, ACHN, 760-P, KETR-3) were obtained from the Chinese Academy of Sciences (Shanghai, China). A498 and ACHN cells were incubated in MEM(10-010-CV, Corning, United States) supplemented with 10% foetal bovine serum (FBS, 16000044, Gibco, United States) and other cells were incubated in RPMI-1640 (10-040-CV, Corning, United States) containing 10% FBS. Cells were grown as a monolayer on plastic cell culture dishes at 37 °C in a humidified atmosphere containing 5% CO2. Sorafenib and RITA (NSC 652287) were purchased from Selleck chemicals (China). MG132 and cycloheximide (CHX) was purchased from APExBIO (United States). The primers used were listed in Supplementary Table 8 and the antibodies used were listed in Supplementary Table 9 .
Mem 10 010 cv
Manufactured by Corning
Sourced in United States
MEM (10-010-CV) is a cell culture medium designed to support the growth and maintenance of a variety of mammalian cell types. It provides the necessary nutrients, vitamins, and other components required for cell proliferation and survival. The medium is formulated to maintain a stable pH and osmotic balance, creating an optimal environment for cell culture applications.
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Lab products found in correlation
Cycloheximide (chx), by Apexbio (2 mentions)
Mg132, by Apexbio (2 mentions)
Rpmi 1640, by Corning (2 mentions)
Penicillin, by American Type Culture Collection (2 mentions)
Streptomycin, by American Type Culture Collection (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Sorafenib, by Selleck Chemicals (1 mentions)
Rpmi 1640 10 040 cv, by Corning (1 mentions)
Streptomycin, by Avantor (1 mentions)
Penicillin, by Avantor (1 mentions)
Fetal bovine serum fbs s11150, by Atlanta Biologicals (1 mentions)
Vero ccl81, by American Type Culture Collection (1 mentions)
Amd3100, by Selleck Chemicals (1 mentions)
Cxcl12, by R&D Systems (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Puromycin, by Merck Group (1 mentions)
Dulbecco modified eagle medium (dmem), by Corning (1 mentions)
6 protocols using mem 10 010 cv
1
RCC Cell Lines Incubation and Treatments
2
Cell Culture Protocols for Viral Studies
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Human astroglial SVG-A derived cells (a kind gift from Walter J. Atwood, Brown University, Providence, RI) were grown at 37 °C and 5% CO2 in Minimum Essential Medium (MEM) (10-010-CV; Corning) supplemented with 10% heat-inactivated fetal bovine serum (FBS, S11150; Atlanta Biologicals), penicillin, and streptomycin (1406-05-9; VWR International). African Green Monkey kidney epithelial MA104 cells (a kind gift from Siyuan Ding, Washington University in St. Louis, St. Louis, MO) were grown at 37 °C and 5% CO2 in Medium 199 supplemented with 10% heat-inactivated FBS. Vero C1008 (Vero 76, clone E6, Vero E6) [American Type Culture Collection (ATCC) CRL-1586] cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% FBS, and penicillin and streptomycin. Vero CCL-81 (ATCC CCL-81) cells were maintained in DMEM supplemented with 10% FBS, 10 mM Hepes pH 7.4, 1% Glutamax, and penicillin/streptomycin.
3
Hypoxic Conditions for Renal Cancer Cell Lines
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The human RCC cell lines (Caki-2, A498, 786-O, ACHN) were obtained from the Chinese Academy of Sciences (Shanghai, China). A498 and ACHN cells were incubated in MEM (10-010-CV, Corning, USA) supplemented with 10% fetal bovine serum (FBS, 16000044, Gibco, USA) and other cells were incubated in RPMI-1640 (10-040-CV, Corning, USA) containing 10% FBS. Cells were grown as a monolayer on plastic cell culture dishes at 37 °C in a humidified atmosphere containing 5% CO2. All experimental cells were cultured under hypoxic conditions unless otherwise specified. Hypoxic conditions were achieved by culturing cells in a sealed chamber after flushing with 1% O2/5% CO2/94% N2, and the cells were cultured in hypoxic conditions for 12 or 24 h to model the hypoxic microenvironment. AMD3100 was purchased from Selleck chemicals (China), 2-Methoxyestradiol was purchased from APExBIO (USA), CXCL12 was purchased from R&D Systems (USA).
4
Human Corneal Epithelial Cell Culture
5
CFTR Variant Expression in CFBE Cells
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CFBE cell lines expressing CFTR variants (WT and R334W) with and without co-expression of the HS-YFP were cultured in minimum essential medium (MEM, #10-010-CV, Corning, VA, USA) supplemented with 10% fetal bovine serum (FBS, #LTI 10270-106, Gibco, Carlsbad, CA, USA) and 2 µg.mL-1 puromycin (#P8833, Sigma-Aldrich, St. Louis, MO, USA) [40 (link),41 (link)]. All cells were maintained at 37 °C and 5% CO2 in a humidified incubator, except for the low-temperature experiments, in which cells were cultured at 27 °C for 24 h.
6
Renal Cell Carcinoma Cell Lines and Endothelial Cell Cultures
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Human RCC cell lines, including OS-RC-2 (cat. no. TCHu40), A498 (cat. no. HTB-44), 786-O (cat. no. TCHu186), ACHN (cat. no. TCHu199), KETR-3 (cat. no. CRL-1161) and human umbilical vein endothelial cells (HUVECs, cat. no. CRL-1730) were obtained from the Chinese Academy of Sciences (Shanghai, China) or the American Type Culture Collection (ATCC). The A498 and ACHN cells were cultured in MEM (10-010-CV, Corning, Inc.) supplemented with 10% fetal bovine serum (FBS, 16000044, Gibco; Thermo Fisher Scientific, Inc.), and the other RCC cells were cultured in RPMI-1640 (10-040-CV, Corning, Inc.) supplemented with 10% FBS. HUVECs were cultured in DMEM (Corning, Inc.) containing 10% FBS. The cells were grown in a single layer on a plastic cell culture dish in humidified air containing 5% CO2 at 37°C. Sunitinib was purchased from Shanghai Selleck Chemicals Co., Ltd. MG132 and cycloheximide (CHX) were obtained from Apexbio Technology, LLC. Recombinant human glutamine peptide loop transferase/QPCT (6368-Zn) was obtained from R&D Systems. Matrigel matrix basement membrane matrix (354234, BD Biosciences) was purchased from Corning, Inc.
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