Plasma was obtained from whole blood, drawn in EDTA tube, by centrifuging at 400xg, for 10 min at room temperature. Plasma samples from IgAN patients and HC were then analyzed with enzyme-linked immunosorbent assay (ELISA) to determine the concentration of the following factors; monocyte chemoattractant protein (MCP-1) (Human CCL2/MCP-1 Quantikine ELISA Kit, R&D Systems, USA), soluble CD14 (Human CD14 Quantikine ELISA Kit, R&D Systems), soluble CD40L (Human CD40 Ligand/TNFSF5 Quantikine ELISA Kit, R&D Systems), B cell activating factor (BAFF) (Human BAFF Quantikine ELISA Kit, R&D Systems), IL-6 (Human IL-6 Quantikine HS ELISA Kit, R&D Systems), Fractalkine (Human CX3CL1/Fractalkine Quantikine ELISA Kit, R&D Systems) and MIP-1 (Human CCL3/MIP-1 alpha Quantikine ELISA Kit, R&D Systems). Catalog number for ELISA kits are shown in S2 Table . The samples were assayed in duplicates and the average values were considered for further analysis. The optical density (OD) in blank well was subtracted from the OD values in samples before calculation of concentrations.
Human cd40 ligand tnfsf5 quantikine elisa kit
Manufactured by R&D Systems
Sourced in United States
The Human CD40 Ligand/TNFSF5 Quantikine ELISA Kit is a quantitative sandwich enzyme immunoassay designed to measure human CD40 Ligand/TNFSF5 levels in cell culture supernates, serum, and plasma.
Automatically generated - may contain errors
Lab products found in correlation
Human ccl2 mcp 1 quantikine elisa kit, by R&D Systems (1 mentions)
Human cd14 quantikine elisa kit, by R&D Systems (1 mentions)
Human cx3cl1 fractalkine quantikine elisa kit, by R&D Systems (1 mentions)
Human baff quantikine elisa kit, by R&D Systems (1 mentions)
Human ccl3 mip 1 alpha quantikine elisa kit, by R&D Systems (1 mentions)
Human il 6 quantikine hs elisa kit, by R&D Systems (1 mentions)
2 protocols using human cd40 ligand tnfsf5 quantikine elisa kit
1
Plasma Biomarker Analysis in IgAN
2
Plasma Immunoassay: EDTA Blood Protocol
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To obtain plasma for immunoassays, EDTA blood was prepared following standard procedures and the samples were stored at –50°C until analysis. Levels of sP-selectin and sCD40L in patients’ plasma were measured using commercially available ELISA tests (respectively, Human P-Selectin/CD62P DuoSet ELISA Quantikine and Human CD40 Ligand/TNFSF5 Quantikine ELISA Kit; R&D Systems, Minneapolis, Minnesota, USA). The intra-assay variability for the lower assay range was less than 10%. The measurement ranges were 0–50 ng/ml for sCD62 and 62–4000 pg/ml for sCD40L.
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