The largest database of trusted experimental protocols

Universal probes one step pcr kit

Manufactured by Bio-Rad

The Universal Probes One-Step PCR kit is a reagent kit designed for performing one-step reverse transcription and polymerase chain reaction (RT-PCR) experiments. The kit includes all the necessary components to conduct RT-PCR in a single reaction, simplifying the experimental workflow.

Automatically generated - may contain errors

2 protocols using universal probes one step pcr kit

1

qPCR Expression Profiling of Mouse Genes

Check if the same lab product or an alternative is used in the 5 most similar protocols
Reverse transcription followed by qPCR was performed in the same reaction using the Universal Probes One-Step PCR kit (Bio-Rad Laboratories) and the Taqman primers Cftr (Mm00445197_m1), Olfm4 (Mm01320260_m1), Wfdc2 (Mm00509434_m1), Il13 (Mm00434204_m1), Csf2 (Mm01290062_m1), Ar (Mm00442688_m1), and Nr3c1 (Mm00433832_m1) (ThermoFisher) on a CFX96 Real Time PCR system (Bio-Rad Laboratories). Messenger RNA levels were normalized to the reference gene Ppib (Mm00478295_m1).
+ Open protocol
+ Expand
2

Gastric Biopsy RNA Isolation and qRT-PCR

Check if the same lab product or an alternative is used in the 5 most similar protocols
RNA used for qRT-PCR and RNAseq was isolated from a 4-mm biopsy specimen of the gastric corpus lesser curvature. RNA was extracted in TRIzol (Thermo Fisher Scientific) and precipitated from the aqueous phase using 1.5 volumes of 100% ethanol. The mixture was transferred to a RNeasy column (Qiagen, Hilden, Germany), and the remaining steps were followed according to the RNeasy kit manufacturer’s recommendations. RNA was treated with RNase-free DNase I (Qiagen) as part of the isolation procedure. Reverse-transcription followed by qPCR was performed in the same reaction using the Universal Probes One-Step PCR kit (Bio-Rad Laboratories, Hercules, CA) and the TaqMan primers (Thermo Fisher Scientific) Cftr (Mm00445197_m1), Olfm4 (Mm01320260_m1), Wfdc2 (Mm00509434_m1), Zfp36 (Mm00457144_m1), Il33 (Mm00505403_m1), and Il13 (Mm00434204_m1) (Thermo Fisher Scientific) on a Quantstudio 6 (Thermo Fisher Scientific). mRNA levels were normalized to the reference gene Ppib (Mm00478295_m1).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!