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Cd28 pe cy5.5 clone cd28 2

Manufactured by Beckman Coulter
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The CD28-PE-Cy5.5 (clone CD28.2) is a flow cytometry reagent. It is a fluorochrome-conjugated monoclonal antibody that binds to the CD28 molecule expressed on T cells.

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Lab products found in correlation

Facsdiva software package, by BD (3 mentions) Cd95 bv605 clone dx2, by BioLegend (3 mentions) Cd62l pe clone sk11, by BD (3 mentions) Lsrii flow cytometer, by BD (3 mentions) Pd 1 bv421 clone eh12.2h7, by BioLegend (2 mentions) Cd45ra pe cy7 clone 5h9, by BD (2 mentions)

3 protocols using cd28 pe cy5.5 clone cd28 2

1

Multicolor Flow Cytometry of Immune Subsets

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Multicolor flow cytometric analysis was performed on whole blood using predetermined optimal concentrations of the following fluorescently conjugated monoclonal antibodies: CD3-allophycocyanin (APC)-Cy7 (clone SP34–2), Ki-67-AF700 (clone B56), CCR7-fluorescein isothiocyanate (FITC) (clone A20), CD45RA-phycoerythrin (PE)-Cy7 (clone 5H9), and CD62L-PE (clone SK11) from BD Biosciences; CD8-Brilliant Violet 711 (BV711) (clone RPA-T8), CD4-BV650 (clone OKT4), and CD95-BV605 (clone DX2) from BioLegend; and CD28-PE-Cy5.5 (clone CD28.2) from Beckman Coulter. Flow cytometric acquisition and analysis of samples were performed on at least 100,000 events on an LSR II flow cytometer driven by the FACSDiva software package (BD Biosciences). Analyses of the acquired data were performed using FlowJo software (version 10.0.4; Tree Star).
Mavigner M., Liao L.E., Brooks A.D., Ke R., Mattingly C., Schoof N., McBrien J., Carnathan D., Liang S., Vanderford T.H., Paiardini M., Kulpa D., Lifson J.D., Dunham R.M., Easley K.A., Margolis D.M., Perelson A.S., Silvestri G, & Chahroudi A. (2021). CD8 Lymphocyte Depletion Enhances the Latency Reversal Activity of the SMAC Mimetic AZD5582 in ART-Suppressed Simian Immunodeficiency Virus-Infected Rhesus Macaques. Journal of Virology, 95(8), e01429-20.
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2

Multicolor Flow Cytometric Immune Profiling

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Multicolor flow cytometric analysis was performed on whole blood and lymph node mononuclear cells using predetermined optimal concentrations of the following fluorescently conjugated monoclonal antibodies: CD3-APC-Cy7 (clone SP34–2), Ki-67-AF700 (clone B56), HLA-DR-PerCP-Cy5.5 (clone G46–6), CCR5-APC (clone 3A9, CCR7-FITC (clone A20 ), CD45RA-PE-Cy7 (clone 5H9), and CD62L-PE (clone SK11) from BD Biosciences; CD8-BV711 (clone RPA-T8), CD4-BV650 (clone OKT4), CD95-BV605 (clone DX2) and PD-1-BV421 (clone EH12.2H7) from Biolegend and CD28-PE-Cy5.5 (clone CD28.2) from Beckman Coulter. Flow cytometric acquisition and analysis of samples was performed on at least 100,000 events on an LSRII flow cytometer driven by the FACSDiva™ software package (BD Biosciences). Analyses of the acquired data were performed using FlowJo™ software (Tree Star, version 10.0.4).
Nixon C.C., Mavigner M., Sampey G.C., Brooks A.D., Spagnuolo R.A., Irlbeck D.M., Mattingly C., Ho P.T., Schoof N., Cammon C.G., Tharp G.K., Kanke M., Wang Z., Cleary R.A., Upadhyay A.A., De C., Wills S.R., Falcinelli S.D., Galardi C., Walum H., Schramm N.J., Deutsch J., Lifson J.D., Fennessey C.M., Keele B.F., Jean S., Maguire S., Liao B., Browne E.P., Ferris R.G., Brehm J.H., Favre D., Vanderford T.H., Bosinger S.E., Jones C.D., Routy J.P., Archin N.M., Margolis D.M., Wahl A., Dunham R.M., Silvestri G., Chahroudi A, & Garcia J.V. (2020). Systemic HIV and SIV latency reversal via non-canonical NF-κB signalling in vivo. Nature, 578(7793), 160-165.
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3

Multicolor Flow Cytometric Immune Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Multicolor flow cytometric analysis was performed on whole blood and lymph node mononuclear cells using predetermined optimal concentrations of the following fluorescently conjugated monoclonal antibodies: CD3-APC-Cy7 (clone SP34–2), Ki-67-AF700 (clone B56), HLA-DR-PerCP-Cy5.5 (clone G46–6), CCR5-APC (clone 3A9, CCR7-FITC (clone A20 ), CD45RA-PE-Cy7 (clone 5H9), and CD62L-PE (clone SK11) from BD Biosciences; CD8-BV711 (clone RPA-T8), CD4-BV650 (clone OKT4), CD95-BV605 (clone DX2) and PD-1-BV421 (clone EH12.2H7) from Biolegend and CD28-PE-Cy5.5 (clone CD28.2) from Beckman Coulter. Flow cytometric acquisition and analysis of samples was performed on at least 100,000 events on an LSRII flow cytometer driven by the FACSDiva™ software package (BD Biosciences). Analyses of the acquired data were performed using FlowJo™ software (Tree Star, version 10.0.4).
Nixon C.C., Mavigner M., Sampey G.C., Brooks A.D., Spagnuolo R.A., Irlbeck D.M., Mattingly C., Ho P.T., Schoof N., Cammon C.G., Tharp G.K., Kanke M., Wang Z., Cleary R.A., Upadhyay A.A., De C., Wills S.R., Falcinelli S.D., Galardi C., Walum H., Schramm N.J., Deutsch J., Lifson J.D., Fennessey C.M., Keele B.F., Jean S., Maguire S., Liao B., Browne E.P., Ferris R.G., Brehm J.H., Favre D., Vanderford T.H., Bosinger S.E., Jones C.D., Routy J.P., Archin N.M., Margolis D.M., Wahl A., Dunham R.M., Silvestri G., Chahroudi A, & Garcia J.V. (2020). Systemic HIV and SIV latency reversal via non-canonical NF-κB signalling in vivo. Nature, 578(7793), 160-165.
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