Renin 3

Manufactured by PerkinElmer

Sourced in France

Renin III is a laboratory instrument designed for the measurement of renin, an enzyme involved in the regulation of blood pressure. The device utilizes an immunoassay-based detection method to quantify renin levels in biological samples.

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Lab products found in correlation

Immulite 2000 xpi, by Siemens (1 mentions) Cortisol ria kit, by Beckman Coulter (1 mentions) Coat a count, by Siemens (1 mentions)

Spelling variants of this product

RENIN III generation (2 citations) Renin III Generation RIA (2 citations)

3 protocols using renin 3

Renin and Prorenin Measurement in Placental and Cell Lysates

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Renin was measured in the placental - and BN16 cell lysates with the Renin III (Cisbio, Gif-sur-Yvette, France) immunoradiometric assay (detection limit 2 pg/mL). Total renin was measured in the lysates with the same assay, after activating prorenin with 10 μmol/L aliskiren (allowing its detection in the assay) at 4 °C for 48 h [45 (link)]. Prorenin in the lysates was calculated by subtracting renin from total renin. Given their low levels, renin and prorenin in the placental perfusates (the latter after its conversion to renin by trypsin) were measured by the more sensitive enzyme-kinetic assay as described before (detection limit 0.05 ng angiotensin I per mL per hour) [46 (link)]. Data were converted to pg/mL given that 1 ng Ang I/mL per hour equals 2.6 pg/mL renin [47 (link)]. AGT in the perfusates was measured as the maximum quantity of Ang I that was generated during incubation with excess recombinant renin (detection limit 0.5 pmol/mL) [46 (link)].

Sun Y., Tan L., Neuman R.I., Broekhuizen M., Schoenmakers S., Lu X, & Danser A.H. (2021). Megalin, Proton Pump Inhibitors and the Renin–Angiotensin System in Healthy and Pre-Eclamptic Placentas. International Journal of Molecular Sciences, 22(14), 7407.

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Steroid Profiling and Hormonal Measurements

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Plasma concentrations of a panel of 15 steroids were measured using a liquid chromatography coupled to tandem mass spectroscopy (LC-MS/MS) assay to avoid the risk of interference between steroids as previously described [14] . The steroids evaluated were pregnenolone, progesterone, corticosterone, deoxycorticosterone (DOC), aldosterone, 17hydroxypregnenolone, 17-hydroxyprogesterone, 11-deoxycortisol, 21-deoxycortisol, cortisol, cortisone, DHEA, delta 4-androstenedione, 11-β-hydroxyandrostenedione, testosterone.
Plasma renin concentration was assessed with commercial kit (Renin III from CisBio). Urinary concentrations of cortisol and plasma concentrations of ACTH were determined using chemiluminescent enzyme immunoassays (Immulite 2000 XPi, Siemens Healthineers).
Routine plasma and urinary aldosterone concentrations were also assessed with a commercial kit (DPC Coat-a-Count assay from Diagnostic Products Corp).

Blanchard A., Brailly Tabard S., Lamaziere A., Bergerot D., Zhygalina V., Lorthioir A., Jacques A., Hourton D., Azizi M, & Crambert G. (2020). Adrenal adaptation in potassium-depleted men: role of progesterone?. Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, 35(11).

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Hormonal and Renal Function Analysis

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Plasma and urine samples were stored at 20 °C until analysis. Plasma renin concentration (PRC) was measured using an immunoradiometric assay (Renin III, CisBio, Codolet, France). Plasma aldosterone concentration (PAC) and 24-h urinary excretion of free aldosterone at pH1 (UAE) were measured using a radioimmunoassay (Coat-a-count, Siemens Medical Solutions Diagnostics, Erlangen, Germany). 24-h urinary free cortisol Excretion (UFCE) was measured by radioimmunoassay (Cortisol RIA kit, Beckman Coulter). The ratio of UAE and UFCE to urinary creatinine excretion was used to standardize the 24hour urine collection. Glomerular filtration rate (GFR) was estimated with the simplified modification of diet in renal disease (MDRD) formula. To account for differences in diet between genotypes and visits, if any, 24-hour urinary sodium and potassium excretion were measured. All biochemical and hormonal assessments were performed blind to the genotype.

Marques P., Courand P.Y., Gouin-Thibault I., Zhygalina V., Bergerot D., Salem J.E., Funck-Brentano C., Loriot M.A., Azizi M, & Blanchard A. (2019). P-glycoprotein influences urinary excretion of aldosterone in healthy individuals. Journal of hypertension, 37(11).

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