Mice were intravenously injected with Annexin-V750 (250 μl, PerkinElmer) 4 hours after MI, 1 hour before imaging. Image acquisition was done using an epifluorescence microscope (OV-110, Olympus) equipped with IV10-ASW 01.01.00.05 software with an image matrix of 512 × 512 and 0.0178 mm × 0.0178 mm pixels. Mice were intravenously injected with TMRE perchlorate (1.5 μl of a 10 mM stock solution diluted in PBS, MedChemExpress) 4.5 hours after MI induction. Carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP, 5 μl diluted in 150 μl of PBS, MedChemExpress) was retro-orbitally injected 15 minutes before TMRE. Oxidative stress was imaged after intravenous injection of CellROX Deep Red Reagent (C10422, Thermo Fisher Scientific, 20 μl diluted in 100 μl of PBS). Hearts were sliced in 1-mm sections for immediate imaging using a Sapphire Biomolecular Imager (Azure Biosystems).
Carbonyl cyanide 4 trifluoromethoxy phenylhydrazone fccp
Manufactured by MedChemExpress
Carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP) is a chemical compound used in research applications. It functions as an ionophore that uncouples oxidative phosphorylation in mitochondria, disrupting the proton gradient and ATP synthesis.
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2 protocols using carbonyl cyanide 4 trifluoromethoxy phenylhydrazone fccp
1
Imaging Myocardial Infarction in Mice
2
Imaging Myocardial Infarction in Mice
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Mice were intravenously injected with Annexin-V750 (250 μl, PerkinElmer) 4 hours after MI, 1 hour before imaging. Image acquisition was done using an epifluorescence microscope (OV-110, Olympus) equipped with IV10-ASW 01.01.00.05 software with an image matrix of 512 × 512 and 0.0178 mm × 0.0178 mm pixels. Mice were intravenously injected with TMRE perchlorate (1.5 μl of a 10 mM stock solution diluted in PBS, MedChemExpress) 4.5 hours after MI induction. Carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP, 5 μl diluted in 150 μl of PBS, MedChemExpress) was retro-orbitally injected 15 minutes before TMRE. Oxidative stress was imaged after intravenous injection of CellROX Deep Red Reagent (C10422, Thermo Fisher Scientific, 20 μl diluted in 100 μl of PBS). Hearts were sliced in 1-mm sections for immediate imaging using a Sapphire Biomolecular Imager (Azure Biosystems).
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