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Amitriptyline ami

Manufactured by Merck Group
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Sourced in United States

Amitriptyline (Ami) is a laboratory product produced by the Merck Group. It is a tricyclic antidepressant compound used in research and pharmaceutical applications. The core function of Amitriptyline is to serve as a reference standard and analytical tool for research and development purposes.

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Lab products found in correlation

Phosphonoacetic acid, by Merck Group (2 mentions) D ribose, by Merck Group (2 mentions) Genistein genis, by Merck Group (2 mentions)

2 protocols using amitriptyline ami

1

Podocyte Immortalization and Treatment

Check if the same lab product or an alternative is used in the 5 most similar protocols
A conditionally immortalized mouse podocyte cell line (Graciously provided by Dr. P. E. Klotman, Division of Nephrology, Department of Medicine, Mount Sinai School of medicine, New York, NY), was cultured and maintained as described previously [23 (link)]. For all experiments, culture medium was replaced with serum-free medium prior to treatments. Podocytes were incubated with D-ribose (25 mM, Sigma, USA) or phosphonoacetic acid (1.0 mM, Sigma Aldrich, USA) for 24 h. Acid ceramidase (AC), AC inducer genistein (genis, 20 μM, Sigma Aldrich, USA) and acid sphingomyelinase (Asm) inhibitor, amitriptyline (Ami, 20 μM, Sigma, St. Louis, MO, USA) were used 30 min prior to treatments [24 (link),25 (link)].
Hong J., Bhat O.M., Li G., Dempsey S.K., Zhang Q., Ritter J.K., Li W, & Li P.L. (2019). Lysosomal regulation of extracellular vesicle excretion during D-ribose-induced NLRP3 inflammasome activation in podocytes. Biochimica et biophysica acta. Molecular cell research, 1866(5), 849-860.
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2

Podocyte Immortalization and Treatment

Check if the same lab product or an alternative is used in the 5 most similar protocols
A conditionally immortalized mouse podocyte cell line (Graciously provided by Dr. P. E. Klotman, Division of Nephrology, Department of Medicine, Mount Sinai School of medicine, New York, NY), was cultured and maintained as described previously [23 (link)]. For all experiments, culture medium was replaced with serum-free medium prior to treatments. Podocytes were incubated with D-ribose (25 mM, Sigma, USA) or phosphonoacetic acid (1.0 mM, Sigma Aldrich, USA) for 24 h. Acid ceramidase (AC), AC inducer genistein (genis, 20 μM, Sigma Aldrich, USA) and acid sphingomyelinase (Asm) inhibitor, amitriptyline (Ami, 20 μM, Sigma, St. Louis, MO, USA) were used 30 min prior to treatments [24 (link),25 (link)].
Hong J., Bhat O.M., Li G., Dempsey S.K., Zhang Q., Ritter J.K., Li W, & Li P.L. (2019). Lysosomal regulation of extracellular vesicle excretion during D-ribose-induced NLRP3 inflammasome activation in podocytes. Biochimica et biophysica acta. Molecular cell research, 1866(5), 849-860.
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