γδ tcr ebiogl3

Manufactured by Thermo Fisher Scientific

The γδ-TCR (eBioGL3) is a laboratory product designed for the detection and analysis of γδ T cells. It provides a tool for researchers to study the role and function of these specialized T cells in various biological and immunological processes.

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EBioGL3 (3 citations) Anti-γδ TCR (eBioGL3) (2 citations)

2 protocols using γδ tcr ebiogl3

Immunological Antibody Staining for Flow Cytometry

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The following antibodies were used for immunofluorescence and immunohistochemistry: CD45R/B220 (RA3-6B2, BD Pharmingen), CD11c (N418, eBioscience), Fc receptor block;anti-CD16/CD32 (2.4G2, BD Pharmingen), CD3 (F7.2.38, Agilent Technologies), von Willebrand factor (vWF) (A0082, Dako). The following antibodies were used for flow cytometry: CD45.1 (A20, eBioscience), CD4 (RM4-5, eBioscience), IL-17A (TC11-18H10, BD Pharmingen), FoxP3 (FJK-16s, eBioscience), RoRγt (Q31-378, BD Pharmingen), CD44 (IM7, eBioscience), IFNγ (XMG1.2, BD Pharmingen), CD8α (53-6.7, eBioscience), γδ-TCR (eBioGL3, eBioscience). Anti-mCD4 (GK1.5, BioXCell) was used for in vivo CD4⁺ T-cell depletion.

Chung A.Y., Li Q., Blair S.J., De Jesus M., Dennis K.L., LeVea C., Yao J., Sun Y., Conway T.F., Virtuoso L.P., Battaglia N.G., Furtado S., Mathiowitz E., Mantis N.J., Khazaie K, & Egilmez N.K. (2014). Oral interleukin-10 alleviates polyposis via neutralization of pathogenic T-regulatory cells. Cancer research, 74(19), 5377-5385.

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Multiparametric Flow Cytometry Analysis

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Cell suspensions were stained with antibodies for mouse studies as follows: FITC-conjugated B220 (RA3-6B2, eBioscience), CD3 (17A2, eBioscience), CD4 (RM4-5, eBioscience), CD5 (53–7.3, eBioscience), CD8α (53–6.7, eBioscience), CD11b (M1/70, eBioscience), CD11c (N418, eBioscience), CD19 (eBio1D3, eBioscience), Gr-1 (RB6-8C5, eBioscience), TCRβ (N57-597, eBioscience), Ter-119 (Ter119, eBioscience), γδTCR (eBioGL3, eBioscience), NK1.1 (PK136, eBioscience), FcεR1 (MAR-1, eBioscience). PE-conjugated ST2 (DJ8, MD Biosciences), PerCP/Cy5.5-cinjugated CXCR4 (Biolegend). APC-conjugated CD90.2 (53–2.1, eBioscience). Alexa Fluor 700-conjugated CD45 (30-F11, eBioscience). BV421-conjugated Ly-6A/E (D7, BD Biosciences). PE-Cyanine 7-conjugated KLRG1 (2F1, BD Biosciences).
To stain GRK2 antigen (R&D systems), cells were fixed and permeabilized after cell surface marker staining. Cells were analyzed by BD LSRII flow cytometer (BD Bioscences) and FlowJo software.

Lai D., Chen W., Zhang K., Scott M.J., Li Y., Billiar T.R., Wilson M.A, & Fan J. (2022). GRK2 regulates group 2 innate lymphoid cell mobilization in sepsis. Molecular Medicine, 28, 32.

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