Log In Sign up
The largest database of trusted experimental protocols

Ha conjugated magnetic beads

Manufactured by Cell Signaling Technology
Visit Supplier

HA-conjugated magnetic beads are a type of lab equipment used for various biological and biochemical applications. These beads are composed of a magnetic core with a surface that has been conjugated with the HA (Hemagglutinin) epitope tag. The magnetic properties of the beads allow for easy separation and manipulation using a magnetic field, while the HA tag enables specific capture and purification of HA-tagged proteins or other molecules.

Automatically generated - may contain errors

Lab products found in correlation

Protease and phosphatase inhibitor, by Merck Group (2 mentions) Igg xp isotype magnetic beads, by Cell Signaling Technology (2 mentions) Ip buffer, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

Magnetic beads (20 citations)

2 protocols using ha conjugated magnetic beads

1

HA-tagged Protein Immunoprecipitation and RNase Treatment

Check if the same lab product or an alternative is used in the 5 most similar protocols
2 × 106 iSLK.KSHV219 cells were transfected with eF4E2-HA, eIF4E1-HA and HIF2α-HA 24 h prior reactivation. Then, 24 h or 48 h post-reactivation, cells were washed with 1X PBS and harvested with IP buffer (ThermoFisher Scientific) containing phosphatase and protease inhibitors (Sigma). 5% of input sample was removed before IP. Then, IgG XP Isotype magnetic beads (Cell Signaling) and HA-conjugated magnetic beads (Cell Signaling) were added to the respective sample following manufacture’s recommendation. The samples containing beads were rotated at 4°C for 6 h. Then beads were washed 4 times prior to RNase treatment. The beads were treated with RNase or left untreated for 20 min at 25°C with rotation. After RNase incubation, the beads were washed 4 additional times and resuspended in 50 μL of 2X Laemmli buffer containing BME, boiled for 5 min and resolved in SDS-PAGE gel.
Méndez-Solís O., Bendjennat M., Naipauer J., Theodoridis P.R., Ho J.J., Verdun R.E., Hare J.M., Cesarman E., Lee S, & Mesri E.A. (2021). Kaposi’s sarcoma herpesvirus activates the hypoxia response to usurp HIF2α-dependent translation initiation for replication and oncogenesis. Cell reports, 37(13), 110144.
+ Open protocol
+ Expand
2

HA-tagged Protein Immunoprecipitation and RNase Treatment

Check if the same lab product or an alternative is used in the 5 most similar protocols
2 × 106 iSLK.KSHV219 cells were transfected with eF4E2-HA, eIF4E1-HA and HIF2α-HA 24 h prior reactivation. Then, 24 h or 48 h post-reactivation, cells were washed with 1X PBS and harvested with IP buffer (ThermoFisher Scientific) containing phosphatase and protease inhibitors (Sigma). 5% of input sample was removed before IP. Then, IgG XP Isotype magnetic beads (Cell Signaling) and HA-conjugated magnetic beads (Cell Signaling) were added to the respective sample following manufacture’s recommendation. The samples containing beads were rotated at 4°C for 6 h. Then beads were washed 4 times prior to RNase treatment. The beads were treated with RNase or left untreated for 20 min at 25°C with rotation. After RNase incubation, the beads were washed 4 additional times and resuspended in 50 μL of 2X Laemmli buffer containing BME, boiled for 5 min and resolved in SDS-PAGE gel.
Méndez-Solís O., Bendjennat M., Naipauer J., Theodoridis P.R., Ho J.J., Verdun R.E., Hare J.M., Cesarman E., Lee S, & Mesri E.A. (2021). Kaposi’s sarcoma herpesvirus activates the hypoxia response to usurp HIF2α-dependent translation initiation for replication and oncogenesis. Cell reports, 37(13), 110144.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!