Human apolipoprotein b elisa kit

Manufactured by Mabtech

The Human apolipoprotein B ELISA kit is a sandwich enzyme-linked immunosorbent assay (ELISA) designed for the quantitative measurement of human apolipoprotein B in biological samples such as serum and plasma.

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Lab products found in correlation

L type tg m, by Fujifilm (1 mentions)

2 protocols using human apolipoprotein b elisa kit

Measurement of ANGPTL3 and ApoB-100

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Concentration of ANGPTL3 was measured utilizing ELISA assay developed by Robciuc et al. [22 (link)]. ApoB-100 was measured with Human apolipoprotein B ELISA kit (Mabtech) according to instructions provided by the manufacturer.

Tikka A., Soronen J., Laurila P.P., Metso J., Ehnholm C, & Jauhiainen M. (2014). Silencing of ANGPTL 3 (angiopoietin-like protein 3) in human hepatocytes results in decreased expression of gluconeogenic genes and reduced triacylglycerol-rich VLDL secretion upon insulin stimulation. Bioscience Reports, 34(6), e00160.

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Differentiated Cell Lipid Metabolism

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24 hour spent culture media from differentiating cells (one million cells per well) at indicated time points (day 0, day 4, day 10 and day 15 post-differentiation) were collected. The amount of ApoB-100 (ng/ml) produced by the cells was measured by the Human Apolipoprotein B ELISA kit (MABTECH, Inc). Triglycerides (mg/ml) were measured using colorimetric assay (L-Type TG M, Wako Diagnostics). Fold change value to day 0 (undifferentiated cells) was obtained by calculating the ratio with that of each time point.

Irudayam J.I., Contreras D., Spurka L., Subramanian A., Allen J., Ren S., Kanagavel V., Nguyen Q., Ramaiah A., Ramamoorthy K., French S.W., Klein A.S., Funari V, & Arumugaswami V. (2015). Characterization of Type I Interferon pathway during Hepatic Differentiation of Human Pluripotent Stem Cells and hepatitis C virus infection. Stem cell research, 15(2), 354-364.

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