Rosa26tdtomato cre reporter

Manufactured by Jackson ImmunoResearch

The Rosa26tdTomato Cre reporter is a genetically engineered mouse line that expresses the Cre recombinase enzyme under the control of the ubiquitously expressed ROSA26 promoter. When crossed with a mouse line containing a floxed target gene, the Cre enzyme will induce recombination, resulting in the expression of a red fluorescent protein (tdTomato) in those cells.

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2 protocols using rosa26tdtomato cre reporter

Standardized Mouse Husbandry and Genetic Strains

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All mouse husbandry and experiments were performed in accordance with Stanford Institutional Animal Care and Use Committee (IACUC) guidelines. All mice were housed in a pathogen-free facility. A maximum of 5 adult mice were housed per cage. Animals were monitored daily for normal husbandry and any health concerns. Cages were kept under a 12-hour light and 12-hour dark cycle. Mouse lines used in this study were: wild type (CD1, Charles River Laboratories, Strain Code #022), Cxcl12^fl/fl (The Jackson Laboratory, B6(FVB)-Cxcl12^tm11Link/J, Stock# 021773), Cxcr4^fl,fl (The Jackson Laboratory, B6.129P2-Cxcr4^tm27zo/J, Stock# 008767), Rosa26^tdTomato Cre reporter (The Jackson Laboratory, B6.Cg-Gt(ROSA)26Sor^{tm9(CAG-TdTomato)Hze}/J, Stock# 007909), Rosa26^ntdTomato Cre reporter (The Jackson Laboratory, B6.Cg-Gt(ROSA)26Sortm75.1^{(CAG-tdTomato}*^)Hze/J, Stock# 025106), Cxcl12-DsRed (The Jackson Laboratory, Cxcl12^tm21Sjm/J, Stock# 022458), pTRE-H2BGFP (The Jackson Tg^{(tetO-mSTm2RJ/GFP)47Efu}/J, Stock# 005104), BmxCreERT2^{22 (link)} All mice in this study were maintained on a mixed background.

D’Amato G., Phansalkar R., Naftaly J.A., Fan X., Amir Z.A., Coronado P.E., Cowley D.O., Quinn K.E., Sharma B., Caron K.M., Vigilante A, & Red-Horse K. (2022). Endocardium-to-coronary artery differentiation during heart development and regeneration involves sequential roles of Bmp2 and Cxcl12/Cxcr4. Developmental cell, 57(22), 2517-2532.e6.

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Genetic Manipulation of Murine Vascular Biology

Cited 2 times

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All mouse colonies were housed and bred in the animal facility at Stanford University in accordance with institutional animal care and use committee (IACUC) guidance. All mouse lines were described earlier: Cx40CreER (Miquerol et al., 2015 (link)), ApjCreER (Chen et al., 2014 (link)), Tie2::eGFP::Claudin5 (Knowland et al., 2014 (link); Lutz et al., 2017 (link)). Cdh5CreER transgenic mice (Wang et al., 2010 (link)). Cxcl12^fl/fl (The Jackson Laboratory, B6(FVB)-Cxcl12^tm1.1Link/J, Stock# 021773), Cxcr4^fl/fl (The Jackson Laboratory, B6.129P2-Cxcr4^tm2Yzo/J, Stock# 008767), Rosa26^TdTomato Cre reporter (The Jackson Laboratory, B6.Cg-Gt(ROSA)26Sor^{tm9(CAG-TdTomato)Hze}/J, Stock# 007909) Rosa26^Zsgreen (The Jackson Laboratory, B6.Cg-Gt(ROSA)26Sor^{tm6(CAG-zsGreen1)Hze}/J, Stock# 007906), and Cxcl12-DsRed (The Jackson Laboratory, Cxcl12^tm2.1Sjm/J, Stock# 022458). For neonatal studies, all Tamoxifen injections to activate Cre activity (6mgs in corn oil) were given intraperitoneally to nursing mothers. Due to mouse genetic inheritance, analyses were compiled from age-matched males and females from multiple litters.

Das S., Goldstone A.B., Wang H., Farry J., D’Amato G., Paulsen M.J., Eskandari A., Hironaka C.E., Phansalkar R., Sharma B., Rhee S., Shamskhou E.A., Agalliu D., de Jesus Perez V., Woo Y.J, & Red-Horse K. (2019). A unique collateral artery development program promotes neonatal heart regeneration. Cell, 176(5), 1128-1142.e18.

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