Ast assay kit c010 2 1

OEA, DMSO, PEG300, and Tween-80 were purchased from Sigma Chemical (USA). Tunicamycin (TM) was purchased from MCE (NJ, USA). Dulbecco's modified Eagle's medium (DMEM) and fetal bovine serum (FBS) were purchased from Viva Cell Biosciences (China). RIPA Lysis Buffer, PMSF, Cell Counting Kit-8 (C0037), DAB Horseradish Peroxidase Color Development Kit, lactate dehydrogenase (LDH), Cytotoxicity Assay Kit, and TUNEL Apoptosis Assay Kit (C1086) were purchased from Beyotime (China). PPARα antibody (Cat: 15540-1-AP), Grp78 antibody (Cat: 11587-1-AP), CHOP antibody (Cat: 15204-1-AP), Caspase 12 antibody (Cat: 55238-1-AP), cleaved-Caspase 3 (Cat: 19677-1-AP), Bcl2 (Cat: 12789-1-AP) antibody, Bax antibody (Cat: 50599-2-Ig), β-actin antibody (Cat: 66009-1-Ig), and HRP-conjugated goat anti-mouse/rabbit IgG (SA00001-1/2) were purchased from Proteintech (China). ALT assay kit (C009-2-1) and AST assay kit (C010-2-1) were purchased from Jiancheng Bioengineering Institute (Nanjing, China). Annexin V PE Apoptosis Detection Kit (559763) was purchased from BD Pharmingen (NJ, USA). Lipofectamine™3000 and TRIzol were purchased from Invitrogen. SYBR Green® Premix was purchased from TaKaRa. Chemiluminescent kit was purchased from NCM Biotech (Suzhou, China).

Mice serum was collected after sacrificed. ALT assay kit (C009-2-1) and AST assay kit (C010-2-1) were obtained from Nanjing Jiancheng Bioengineering Institute (Nanjing, China) and used to measure serum ALT and AST levels according to the manufacturer’s instructions.