Formic acid of lc ms grade

Paeoniae Radix Alba (batch number: 18061201, source: Anhui China) and Glycyrrhizae Radix et Rhizoma Praeparata Cum Melle (batch number: 180518; source: Neimenggu China), which were authenticated by Professor Jingming Jia (Department of TCM, Shenyang Pharmaceutical University, Shenyang, China), were purchased from Guoda pharmacy (Shenyang, China). The primary source of reference compounds (purity > 98%), including benzoyl paeoniflorin, albiflorin, ononin, and glycyrrhizic acid, was Shanghai Yuanye Bio-Technology Co., Ltd (Shanghai, China), while gallic acid, liquiritin, paeoniflorin, and liquiritin apioside were obtained from the National Institute for the Control of Pharmaceutical and Biological Products (Beijing, China). Moreover, acetonitrile of HPLC grade and formic acid of LC-MS grade were obtained from Fisher Scientific (Fair Lawn, NJ, USA); purified water was then purchased from Wahaha (Hangzhou, China).

The purchase of key ingredients including, Aurantii fructus immaturus (batch number: 170 901; source: Sichuan China), Rheum palmatum L. (batch number: 20 181 101; source: Gansu China), Magnoliae Officmalis Cortex (batch number: 181 001; source: Sichuan China) and Notopterygii Rhizoma Et Radix (batch number: 13 112 501; source: Sichuan China) was made from Guoda pharmacy (Shenyang, China), which followed identification by Professor Jingming Jia (Department of TCM, Shenyang Pharmaceutical University, Shenyang, China). The key source of the reference compounds (purity > 98%), including sennoside B, nodakenin, narirutin, sennoside A, neohesperidin, naringenin, hesperetin, nobiletin was Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China); naringin, hesperidin and honokiol was attained from the National Institute for the Control of Pharmaceutical and Biological Products (Beijing, China). In addition, acetonitrile of HPLC grade, together with formic acid of LC-MS grade was attained from Fisher Scientific (Fair Lawn, NJ, USA), followed by attaining the purified water from Wahaha (Hangzhou, China).