Mouse experiments were performed in strict accordance with the guidelines of the European Community (86/609/EEC) and the French National Committee (87/848) for the care and use of laboratory animals. The study plan was approved by the Institutional Review Board at the Animal Facility of the Institute de Génétique Moléculaire de Montpellier (IGMM) and the Regional Ethics Committee for Animal Experimentation of Languedoc-Roussillon (agreement n° CEEA-LR-12087).
The DSS experiments described in Fig.1 were performed at Amylgen (In vivo drug discovery, Montferrier sur Lez, France), whereas those described in Figs 2 , 3 and 6 were done at the animal facility of IGMM. For DSS-induced colitis, age- and sex-matched C57BL6 mice received one cycle of 2.5% (w/v) DSS administered via drinking water. Mice received 40 mg/kg ABX464 suspended in MC or MC only via gavage when indicated. For antagonizing IL-22 in vivo, mice received either blocking IL-22 antibody (Ebioscience; clone IL-22JOP) or isotype control (Ebioscience; clone eBR2a).
The DSS experiments described in Fig.