Item imaging platform software

Powder X-ray Diffraction (XRD) measurements were carried out to determine the iron oxide phases and the crystal sizes using a Bruker D8 Advance diffractometer equipped with Cu-Kα (1.54178 Å) radiation and operating in $\theta$ - $\theta$ Bragg–Brentano geometry at 40 kV and 40 mA, covering a [25°;70°] range, using 0.02° size step and acquisition time of 1 s/step. A CM12 PHILIPS Transmission Electron Microscope (TEM) operating at 100 kV using an LaF6 source was used to assess the MNPs core sizes and shapes. A dilute toluene dispersion of MNPs was drop-casted onto 200 mesh carbon-coated copper grids to prepare the samples. The recorded micrographs were processed by the iTEM Imaging. Platform software (Olympus), and were further analysed with the FIJI open software. The mean diameter and size distribution of the sample was obtained from a statistical analysis over 200 MNPs.

Monolayer cell cultures of LNCAP cells and platelets were conducted in 8 wells Permanox Lab-Tek^® Chamber Slides (NUNC) and fixed at 4 °C in 1.5% glutaraldehyde, 1% formaldehyde, 0.05 M cocodilate buffer. Fixed cells were post-fixed in 1% osmium tetroxide for 1 hour at 4 °C, washed in distilled water, and treated with 0.15% tannic acid and 2% uranyl acetate. Then, dehydration through graded alcohols and propylene oxide, and then EMbedding in EMbed (Electron Microscopy Sciences) was done. Ultrathin sections (50-70 nm) were stained with 1% uranyl acetate and lead citrate 22 (link). Samples were prepared and examined in a Transmission Electron Microscope using the Libra 120 (Zeiss) ITEM Imaging Platform Software (Olympus) at the Centre for Scientific Instrumentation (CIC) of the University of Granada.

For electron microscopy studies, brains were processed as previously described. In brief, brain samples were fixed by immersion in 4% glutaraldehyde, buffered to pH 7.3 with Millonig's fluid, post‐fixed in 1% osmium tetroxide in the same buffer, and dehydrated in acetone for embedding in Araldite (Sigma). Ultrathin sections were obtained using an OM‐U3 ultramicrotome (Reichert, Vienna, Austria), double‐stained with uranyl acetate and lead citrate, and examined using a JEM 1010 electron microscopy (JEOL, Tokyo, Japan) at the ICTS Spanish National Centre for Electron Microscopy. Images were recorded using a MegaView G2 camera using iTEM Imaging Platform software (Olympus, Münster, Germany).