Tissue culture treated plates

Manufactured by BD

Tissue culture treated plates are a type of laboratory equipment designed for cell and tissue culture applications. These plates provide a treated surface that promotes the attachment and growth of a variety of cell types. The surface treatment enhances the cell-to-surface interaction, enabling researchers to maintain and propagate cells in controlled in vitro environments.

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Lab products found in correlation

Trizol reagent, by Thermo Fisher Scientific (1 mentions) Tripalmitoylated lipopeptide pam3csk4, by InvivoGen (1 mentions) Relesr, by STEMCELL (1 mentions) Matrigel hesc qualified matrix, by Corning (1 mentions) Crystal violet, by Merck Group (1 mentions) Collagen 1, by BD (1 mentions)

Spelling variants of this product

Tissue culture plates (45 citations) Tissue-culture treated adherent 6-well plates (2 citations) 24-well tissue-culture-treated plates (2 citations) Tissue culture-treated 96-well plates (2 citations) Tissue culture‐treated flat‐bottom 96‐well plates (2 citations) Tissue-culture treated adherent 10cm plates (2 citations) 24-well flat bottom tissue culture treated plates (2 citations)

4 protocols using tissue culture treated plates

Cell Culture Plate Comparison

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Corning, Costar Tissue Culture treated plates, Catalog#: 3513 and BD Falcon, Tissue Culture treated plates, Catalog#: 353,043.

Chirila F.V., Xu G., Fontaine D., Kern G., Khan T.K., Brandt J., Konishi Y., Nebe-von-Caron G., White CL I.I.I, & Alkon D.L. (2022). Morphometric imaging biomarker identifies Alzheimer’s disease even among mixed dementia patients. Scientific Reports, 12, 17675.

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In vitro Immune Training of Bovine Monocytes

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In vitro immune training was performed as described previously [16 (link), 34 (link)] (Fig 1A). Briefly, isolated bovine monocytes were plated in a total volume of 200 μl/well of cRPMI, at a concentration of 1x10⁵ cells/well in flat-bottom, 96-well tissue-culture-treated plates (Falcon). Monocytes were incubated with culture medium only as a negative control. Cells were infected at a 5:1 multiplicity of infection (MOI) with BCG strain Danish for 24 hours at 37° C/5% CO₂, washed once with warm culture media, resuspended in cRPMI and incubated for 6 days. The media was replaced once, at day 3. After the incubation, the media was removed and cells were re- stimulated with cRPMI, E. coli lipopolysaccharide (LPS; serotype 055:B5; Sigma-Aldrich, 1 μg/mL) or tripalmitoylated lipopeptide (Pam3CSK4; InvivoGen, 10 μg/mL) for 4 hours (mRNA expression) or 72 hours (protein expression). After the indicated times, cells were pelleted, supernatants collected for cytokine measurement and cells lysed with Trizol Reagent (Invitrogen, Life Technologies) for mRNA transcription assessment and stored at -80°C.

Guerra-Maupome M., Vang D.X, & McGill J.L. (2019). Aerosol vaccination with Bacille Calmette-Guerin induces a trained innate immune phenotype in calves. PLoS ONE, 14(2), e0212751.

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Maintenance and Passaging of Human iPSCs

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We used two human iPSC lines, one derived from a 43-year-old female (MGH2069) and the other derived from a 63-year-old male (GM08330). iPSCs were cultured on tissue culture-treated plates (Falcon) coated with Matrigel hESC-Qualified Matrix (Corning) in Essential 8 medium (E8, Gibco) supplemented with penicillin/streptomycin (P/S, Gibco). For routine culture, cells were maintained on 6-well plates in a humidified incubator at 37°C with 5% CO₂ and passaged every 3–4 days (at ∼70–80% confluence). For passaging, cells were washed with Dulbecco’s Phosphate-Buffered Saline lacking calcium and magnesium (DPBS, Gibco), treated with ReLeSR (Stemcell Technologies) for 7 min at 37°C, resuspended in working medium supplemented with 10 μM ROCK inhibitor (RI, Y-27632, Biological Industries), and plated into new wells after pipetting to reduce the size of cell aggregates. Working medium (E8 + P/S) was refreshed daily. For thawing, cells were resuspended in working medium supplemented with 10 μM RI and plated.

Nuttle X., Burt N.D., Currall B., Moysés-Oliveira M., Mohajeri K., Bhavsar R., Lucente D., Yadav R., Tai D.J., Gusella J.F, & Talkowski M.E. (2023). Parallelized engineering of mutational models using piggyBac transposon delivery of CRISPR libraries. Cell Reports Methods, 4(1), 100672.

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Colony Formation Assay with TGFBI

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For colony formation, cells (1.5×10³/well) were seeded into 96 well tissue-culture treated plates (BD Falcon) and colony growth was measured by crystal violet (Sigma, St Louis, MO) staining after ten days, and then photographed. Fresh recombinant TGFBI (R&D systems) (50 µg/ml) or BSA (5%) was added at every 72 hours, in appropriate experiments. If appropriate, Collagen-I (BD Biosciences) was either added during the start of the experiment or used to coat the plates at 10 µg/ml. Each experiment consisted of three replicates and was repeated three times.

Rudra-Ganguly N., Lowe C., Mattie M., Chang M.S., Satpayev D., Verlinsky A., An Z., Hu L., Yang P., Challita-Eid P., Stover D.R, & Pereira D.S. (2014). Discoidin Domain Receptor 1 Contributes to Tumorigenesis through Modulation of TGFBI Expression. PLoS ONE, 9(11), e111515.

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