Log In Sign up
The largest database of trusted experimental protocols

Ab253217

Manufactured by Abcam
Visit Supplier
Sourced in Austria

Ab253217 is a lab equipment product offered by Abcam. It is a device used for the purpose of handling and manipulating samples during laboratory procedures. The core function of this product is to facilitate the efficient and controlled handling of materials in a laboratory setting.

Automatically generated - may contain errors

Lab products found in correlation

Ab181421, by Abcam (1 mentions) Ab274394, by Abcam (1 mentions) Ab102531, by Abcam (1 mentions) Ab253219, by Abcam (1 mentions) Ab102523, by Abcam (1 mentions) Emmpo, by Thermo Fisher Scientific (1 mentions) Bms213inst, by Thermo Fisher Scientific (1 mentions) Synergy ht, by Agilent Technologies (1 mentions)

5 protocols using ab253217

1

Cytokine Profiling in Cell Culture

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cell culture supernatant was collected where the contents of IL-10, IL-12, iNOS, and TNF-α were detected using IL-10 (KET6019, Abbkine), IL-12 (KET6020, Abbkine), iNOS (ab253217, Abcam) and TNF-α (KET6032, Abbkine) kits, respectively.
Deng J, & Ke H. (2022). Overcoming the resistance of hepatocellular carcinoma to PD-1/PD-L1 inhibitor and the resultant immunosuppression by CD38 siRNA-loaded extracellular vesicles. Oncoimmunology, 12(1), 2152635.
+ Open protocol
+ Expand
2

Quantifying Human and Rat NOS2 by ELISA

Check if the same lab product or an alternative is used in the 5 most similar protocols
The concentrations of human NOS2 (cat. no. ab253217; Abcam) and rat NOS2 (cat. no. SBJ-R0012; SenBeiJia Biological Technology) were measured by ELISA according to the protocols provided by the kit manufacturers.
Zhao Y., Guo L., Tian J, & Wang H. (2021). Downregulation of microRNA-185 expression in diabetic patients increases the expression of NOS2 and results in vascular injury. Experimental and Therapeutic Medicine, 22(6), 1458.
+ Open protocol
+ Expand
3

Measurement of Inflammatory Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
The concentration of inducible nitric oxide synthase (iNOS; cat. no. ab253217; Abcam), TNF-α (cat. no. ab181421; Abcam), CDl63 (cat. no. ab274394; Abcam), arginase-1 (Arg-1; cat. no. BMS2216; Thermo Fisher Scientific, Inc.) and mannose receptor (MR; cat. no. ab277420; Abcam) in the culture medium was measured by ELISA-kits according to the manufacturer's instructions, respectively.
Gao J., Suo S., Li J., Wang C., Deng R., Hu Y, & Zhang C. (2023). IGFBP‑rP1 affects the proliferation, apoptosis and macrophage polarization of endometrial cancer cells by regulating the PI3K/AKT pathway. Experimental and Therapeutic Medicine, 25(4), 169.
+ Open protocol
+ Expand
4

Biomarker Quantification Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
The amylase (#ab102523, Abcam), myeloperoxidase level (#EMMPO, Thermo Fisher Scientific), trypsin (#ab102531, Abcam), 4-HNE (#STA-838, Cell Biolabs), 8-OHG (#K4160, BioVision), IL-6 (#EH2IL6 and #KMC0061, Thermo Fisher Scientific), and NOS2 (#ab253219 and #ab253217, Abcam) concentrations or activity in the samples were measured using ELISA kits.
Dai E., Han L., Liu J., Xie Y., Zeh H.J., Kang R., Bai L, & Tang D. (2020). Ferroptotic damage promotes pancreatic tumorigenesis through a TMEM173/STING-dependent DNA sensor pathway. Nature Communications, 11, 6339.
+ Open protocol
+ Expand
5

Quantification of Inflammatory Markers in Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols
IL-6, iNOS, VEGF, PGE2, and ADMA levels were assessed on BPH-1 and/or H5V cell lysates using enzyme-linked immunosorbent assays (ELISA) (respectively, BMS213INST, eBioscience, Vienna, Austria; ab253217, Abcam, Cambridge, U.K.; KHG0111, KHL1701, Invitrogen, Waltham, MA, USA; DLD-Diagnostika, Hamburg, Germany) according to the manufacturer’s instructions. The absorbance was measured using a microplate reader (Biotek Synergy-HT, Winooski, VT, USA). The results are expressed as pg/mL for IL-6, VEGF, and PGE2, nmoles/mL for ADMA, and pg/mg prot for iNOS.
Consoli V., Burò I., Gulisano M., Castellano A., D’Amico A.G., D’Agata V., Vanella L, & Sorrenti V. (2023). Evaluation of the Antioxidant and Antiangiogenic Activity of a Pomegranate Extract in BPH-1 Prostate Epithelial Cells. International Journal of Molecular Sciences, 24(13), 10719.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!