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4 protocols using sabouraud dextrose agar media

1

Antifungal Evaluation of Silver Nitrate

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Silver nitrate was purchased from MERK India Ltd., Mueller–Hinton Media and Sabouraud dextrose agar media were procured from HIMEDIA India Ltd., and Candida albicans MTCC 183 was obtained from the Institute of Microbial Technology, Chandigarh, India, and all other materials used were of analytical grade.
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2

Citronella Essential Oil Antimicrobial and Anticancer Evaluation

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Citronella essential oil (CITEO) was procured from Indian essential oil (New Delhi, India), while nutrient media and Sabouraud dextrose agar media was purchased from HiMedia (Mumbai, India). Tween 80, MTT reagent, Dimethyl sulfoxide (DMSO), and Camptothecin (CPT) were purchased from Sigma Merck (St Louis, MO, USA). The other reagents or chemicals used in the study were analytically graded. Staphylococcus aureus (ATCC 25923) and Candida albicans (ATCC 10231) were collected from the Department of Pharmaceutics, College of Pharmacy, Prince Sattam Bin Abdulaziz University (PSAU), Al-Kharj. Solvents and other chemicals were also purchased from Sigma Merck. The human skin adenocarcinoma cell line A431 was obtained as gift from Dr. Mohammad Raish, Department of Pharmaceutics, King Saud University (Riyadh, Saudi Arabia).
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3

Natamycin-Loaded Chitosan Nanoparticles

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Natamycin was procured from AKUMS Drugs & Pharmaceuticals Ltd. (Haridwar, UK, India). Dicetyl phosphate (DCP), chitosan (low molecular weight), triethylamine, iodomethane, and N-methylpyrrolidone (NMP) were purchased from Sigma-Aldrich, India. Sterile blank disks, MOPS (3-(N-morpholino)propanesulfonic acid) buffer, Sabouraud dextrose agar media, RPMI (Roswell Park Memorial Institute) media, and cholesterol (CHOL) were procured from HiMedia, Mumbai, India. Span 60 (Sp60) and sodium hydroxide were purchased from CDH (Central Drug House; New Delhi, India). Sodium chloride and acetone were purchased from Fisher Scientific India. Cellulose acetate membrane filter (syringe filter) was purchased from Chromatopak. Methanol and chloroform (Merck Life Science Pvt. Ltd., Mumbai, India), phosphotungstic acid (LobaChemie), sodium hydrogen carbonate, and calcium chloride (CDH, New Delhi) were obtained from the departmental chemical store. The marketed anti-keratitis eye drops, Nataforce (5% w/v, Mankind), was purchased from Netmeds. Ultrapure water (Millipore, Bedford, MA) was used throughout the studies. All other chemicals were of the highest grade commercially available.
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4

Formulation and Evaluation of Rutin-Loaded Gel

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Rutin and silver nitrate were purchased from Sigma Aldrich, USA. Acacia gum and Carbopol 940 were purchased from Loba Chemie (P) Ltd., Mumbai. Di-sodium hydrogen phosphate dihydrate (Sigma-Aldrich) and sodium dihydrogen orthophosphate dihydrate (Merck) were used to prepare 0.1 M phosphate buffer (PB) for buffer solution preparation. Nutrient agar media, Sabouraud dextrose agar media, nutrient broth, and Sabouraud dextrose broth were purchased from Himedia, India. Analytical grade solvents and chemicals were used in their purest form and were not further purified.
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