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Anti ho 1 pab

Manufactured by Enzo Life Sciences
Sourced in United States

Anti-HO-1 pAb is a polyclonal antibody that specifically recognizes the Heme Oxygenase-1 (HO-1) protein. HO-1 is an enzyme involved in the degradation of heme and the production of bilirubin, carbon monoxide, and iron. This antibody can be used in various research applications for the detection and analysis of HO-1 expression.

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3 protocols using anti ho 1 pab

1

Cellular Response to Inflammatory Stimuli

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Dulbecco’s modified Eagle medium (DMEM), fetal bovine serum (FBS), L-glutamine/penicillin/streptomycin, and anti-α-tubulin monoclonal antibodies (mAbs) were purchased from Invitrogen (Thermo Fisher Scientific, Waltham, MA, USA). LPS and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were bought from Sigma-Aldrich (St. Louis, MO, USA). Anti-iNOS, COX2, and Nrf2 polyclonal antibodies (pAbs) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). An anti-HO-1 pAb was purchased from Enzo (Farmingdale, New York, NY, USA). The anti-phospho-p65 (Ser536) mAb was purchased from Cell Signaling (Danvers, MA, USA). Horseradish peroxidase (HRP)-conjugated donkey anti-rabbit immunoglobulin G (IgG) and sheep anti-mouse IgG were purchased from Amersham (Buckinghamshire, U.K.). Enhanced chemiluminescence (ECL) Western blotting detection reagent and Hybond-P polyvinylidene difluoride (PVDF) blotting membranes were purchased from GE Healthcare Life Sciences (Waukesha, WI, USA).
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2

Flavonoid Antioxidant Mechanisms Elucidation

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Dulbecco's modified Eagle's medium (DMEM)/F-12 and fetal bovine serum (FBS) were purchased from Invitrogen (Carlsbad, CA, USA). 5, 8-Dihydroxy-4′,7-dimethoxyflavone (DDF, Figure 1) was kindly provided by the Natural Products Laboratory of Dr. Yann-Lii Leu (Chang Gung University, Taiwan). Hybond C membrane and enhanced chemiluminescence (ECL) Western blotting detection system were purchased from GE Healthcare Biosciences (Buckinghamshire, England, UK). Antibodies against phospho-p38 and phospho-Nrf2 were purchased from Cell Signaling (Danvers, MA). Actinomycin D (Act.D), cycloheximide (CHI), N-acetyl cysteine, SP600125, GSH, trolox, diphenyleneiodonium chloride (DPI), PD98059, and p38 inhibitor VIII (p38i VIII) were purchased from Biomol (Plymouth Meeting, PA). Anti-β-actin antibody was purchased from Biogenesis (Boumemouth, UK). Anti-GAPDH (#MCA-1D4) was obtained from EnCor Biotechnology (Gainesville, FL). Anti-HO-1 pAb and Glutathione (GSSG/GSH) detection kits were purchased from Enzo Life Sciences (Farmingdale, NY). CTGF antibody (sc-25440) was purchased from Santa Cruz (Santa Cruz, CA, USA). Mitotempol, dihydroethidium (DHE), and CM-H2DCFDA were purchased from Molecular Probes (Eugene, OR, USA). SDS-PAGE reagents were purchased from MDBio Inc. (Taipei, Taiwan). Thrombin and other chemicals were purchased from Sigma (St. Louis, MO, USA).
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3

Columbianadin Inhibits Inflammatory Pathways

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Columbianadin (CBN, >98%) was purchased from ChemFaces Biochem, Wuhan, Hubei, China. Dimethyl sulfoxide (DMSO), PD98059, SP600125, BAY11-7082, and DMPO were purchased from Sigma (St Louis, MO, USA). The antibodies against phospho-p38 MAPK Ser182 (pAb), IκBα (44D4), phospho-c-JNK (Thr183/Tyr185), p38 MAPK, NF-κB p65 (mAb), phospho-p44/p42 ERK (Thr202/Tyr204), and phospho-NF-κB p65 (Ser536) pAb were all purchased from Cell Signaling (Beverly, MA, UAS). Anti-HO-1 pAb was purchased from Enzo (Farmingdale, New York, USA). The monoclonal antibody against α-tubulin was derived from NeoMarkers (Fremont, CA, USA). CBN was dissolved in 0.1% DMSO. All other chemicals and reagents used in this study were commercially purchased from Sigma until specified otherwise.
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