1,2-dioleoyl-3-trimethylammonium-propane (DOTAP), Cholesterol and Maleimide-terminated PEG-DSPE (Mal-PEG) were purchased from Avanti Polar- lipids Inc. (Birmingham, AL, USA). Protamine sulfate salt Grade X, trehalose dihydrate and HPLC grade chloroform were obtained from Sigma Chemical Co. (St. Louis, MO, USA). PLGA and PLGA-PEG were purchased from Boehringer Ingelheim (Germany). Lipofectamine RNAiMAX transfection reagent was purchased from Invitrogen. Fetal bovine serum albumin (BSA), Dulbecco’s modified Eagle’s medium (DMEM) and penicillin/streptomycin antibiotics were purchased from Gibco, Invitrogen Corp. (Carlsbad, CA, USA). β-actin antibody was purchased from Sigma Chemical Co. (St. Louis, MO, USA), anti-mouse and anti-rabbit IgG HRP labeled secondary antibody were purchased from GE Healthcare (Little Chalfont, UK). GAPDH siRNA and aptamers were purchased from Life Technologies (Carlsbad, CA). Anti-P-gp antibody was purchased from Pierce, Thermo Scientific (Waltham, MA). All other reagents were of analytical grade and were supplied by Sigma Chemical Co. (St. Louis, MO, USA).
Plga peg
Manufactured by Boehringer Ingelheim
Sourced in Germany
PLGA-PEG is a biodegradable copolymer composed of poly(lactic-co-glycolic acid) (PLGA) and polyethylene glycol (PEG). It is used as a component in various laboratory applications, such as drug delivery systems and tissue engineering.
Automatically generated - may contain errors
Lab products found in correlation
Hplc grade chloroform, by Merck Group (1 mentions)
Trehalose dihydrate, by Merck Group (1 mentions)
β actin antibody, by Merck Group (1 mentions)
Lipofectamine rnaimax transfection reagent, by Thermo Fisher Scientific (1 mentions)
Protamine sulfate salt grade x, by Merck Group (1 mentions)
Poloxamer 188 lutrol f68, by BASF (1 mentions)
N hydroxysuccinimide, by Merck Group (1 mentions)
N n dimethylformamide (dmf), by Scharlab (1 mentions)
N diisopropylcarbodiimide, by Merck Group (1 mentions)
N diisopropylethylamine, by Merck Group (1 mentions)
2 protocols using plga peg
1
Lipid-Based Nanocarrier Formulation
2
Multifunctional PLGA-PEG Nanoparticles
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
PLGA with a 50:50 monomer ratio (Resomer® RG 503H, 34 kDa, viscosity 0.32–0.44 dL/g) together with the PLGA 50:50 and PEG copolymer (Resomer® RGP type d5055 [PLGA-PEG®]) were purchased from Boehringer Ingelheim (Ingelheim, Germany). Maleimide-PEG-NH2 (2,000 Da) was obtained from Jenkem (Beijing, People’s Republic of China). Methoxy-PEG-NH2, N-hydroxysuccinimide (NHS), ethyl-3-(3-dimethylaminopropyl) carbodiimidey-drochloride (EDC), N-diisopropylethylamine (DIEA), N-diisopropylcarbodiimide, 2-(1H-7-azabenzotriazol-1-yl)-1.1.3.3-tetramethyluroniumhexafluorophosphate (HATU), 1-hydroxybenzotriazole, and FB were purchased from Sigma (St Louis, MO, USA). Amino acids and NovaSyn TGR resin were purchased from Novabiochem (Hohenbrunn, Germany). N,N-Dimethylformamide (DMF) was obtained from Scharlau (Barcelona, Spain). Poloxamer 188 (Lutrol® F68) was from BASF (Barcelona, Spain) and 5(6)-carboxytetramethylrhodamine (Rho) was obtained from Merck (Hohenbrunn, Germany). Double-distilled water was used after filtration in a Millipore system. All other chemicals and reagents used in the study were of analytical grade.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!