A total of 10 ng of the extracted RNA was subjected to reverse transcriptase PCR using the TaqMan MicroRNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA, USA), according to the manufacturer’s instructions. Real-time PCR amplification was performed with TaqMan MicroRNA Assays specific for miR-223-3p (Assay ID: 002295; Cat. Num.: 4427975), miR-24-3p (Assay ID: 000402; Cat. Num.: 4427975), miR-1-3p (Assay ID: 002222; Cat. Num.: 4427975), miR-133a-3p (Assay ID: 002246; Cat. Num.: 4427975), miR-133b-3p (Assay ID: 002247; Cat. Num.: 4427975), and miR-206-3p (Assay ID: 000510; Cat. Num.: 4427975) (Applied Biosystems, Foster City, CA, USA) according to the manufacturer’s instructions. miRNA levels were normalized to miR-16-5p (Assay ID: 000391; Cat. Num.: 4427975) for patients’ serum samples, to snoR-135 (Assay ID: 001230; Cat. Num.: 4427975) for mouse tissues, and to the spike-in control cel-miR-39 (Assay ID: 000200; Cat. Num.: 4427975) for the encapsulated miRNA levels in the small EVs (Applied Biosystems, Foster City, CA, USA). Data analysis was performed with SDS 2.4 Real-Time PCR data analysis software (Applied Biosystems, Foster City, CA, USA).
Sds 2.4 real time pcr data analysis software
Manufactured by Thermo Fisher Scientific
Sourced in United States
The SDS 2.4 Real-Time PCR data analysis software is a tool designed to analyze and interpret data generated by real-time PCR (polymerase chain reaction) experiments. It provides users with the necessary functions to process, visualize, and report the results of their real-time PCR studies.
Automatically generated - may contain errors
2 protocols using sds 2.4 real time pcr data analysis software
1
Quantification of Circulating miRNA Biomarkers
2
Serum miRNA Expression Analysis
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Following serum collection, total RNA, including miRNAs, was extracted from serum samples using the mirVana PARIS Kit (Applied Biosystems), according to the manufacturer’s instructions. A total of 10 ng of the extracted RNA was subjected to Reverse Transcriptase PCR using the TaqMan MicroRNA Reverse Transcription Kit (Applied Biosystems), according to the manufacturer’s instructions. Real-Time PCR amplification was performed using TaqMan MicroRNA Assays to measure miRNAs levels. miRNA detection assays specific for miR-1, miR-133a, miR-133b and miR-206 (Applied Biosystems) were carried out according to the manufacturer’s instructions. miRNA expression was normalized to the miR-16 (Applied Biosystems). Data analysis was performed using the SDS 2.4 Real-Time PCR data analysis software (Applied Biosystems).
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