3 4 5 dimethylthiazol 2 5 diphenyltetrazolium bromide mtt

Manufactured by Beyotime

Sourced in China

3-(4,5-dimethylthiazol)-2,5-diphenyltetrazolium bromide (MTT) is a chemical compound commonly used in cell viability and proliferation assays. It is a yellow tetrazolium salt that can be reduced to purple formazan crystals by metabolically active cells. The amount of formazan produced is directly proportional to the number of viable cells, making MTT a useful tool for measuring cell growth and cytotoxicity.

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Lab products found in correlation

Trypsin, by Thermo Fisher Scientific (1 mentions) Rhodamine 6g, by Sinopharm (1 mentions) Dulbecco modified eagle medium dmem, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Beyotime (1 mentions) Sodium nitrite, by Sinopharm (1 mentions) N n dimethylformamide (dmf), by Sinopharm (1 mentions) Chloroauric acid, by Maclin Biochemical Technology (1 mentions) Sodium hydroxide (naoh), by Sinopharm (1 mentions)

2 protocols using 3 4 5 dimethylthiazol 2 5 diphenyltetrazolium bromide mtt

Synthesis and Cytotoxicity Evaluation of 2,3-Diamino Pyridine Derivative

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2,3-Diamino pyridine (PDA) was bought from Aladdin Reagent Company (Shanghai, China). Sodium nitrite, N,N-dimethylformamide (DMF) and Rhodamine 6G (99%) were purchased from Sinopharm Chemical Reagent Co. Ltd (Shanghai, China). All the chemicals were analytically pure and used without further purification. Deionized water (18.2 MΩ cm resistivity) was used throughout this study. Trypsin, Fetal bovine serum (FBS) and Dulbecco modified Eagle medium (DMEM) were ordered from GIBCO. DAPI and 3-(4,5-dimethylthiazol)-2,5-diphenyltetrazolium bromide (MTT) were ordered from Beyotime Institute of Biotechnology (Shanghai, China). HeLa cells and A549 cells were ordered from the Cell Bank of Chinese Academy of Sciences (Shanghai, China).

Zan M., Li C., Liao F., Rao L., Meng Q.F., Xie W., Chen B., Qie X., Li L., Wang L., Dong W.F, & Liu W. (2020). One-step synthesis of green emission carbon dots for selective and sensitive detection of nitrite ions and cellular imaging application. RSC Advances, 10(17), 10067-10075.

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Chloroauric Acid and Peptide Synthesis

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Chloroauric acid was purchased from Macklin Biochemical Co., Ltd. (Shanghai, China). The CWR11 peptide (CWFWKWWRRRRR) was purchased from China Peptides Co., Ltd. (Shanghai, China). Sodium hydroxide (NaOH) was obtained from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). 3-(4,5)-Dimethylthiazol-2,5-diphenyltetrazolium bromide (MTT) was purchased from Beyotime Biotechnology Co., Ltd. (Shanghai, China). Ultrapure water (18.2 MΩ) was used throughout the experiments.

Shen Y., Zheng C., Wu Q., Wu Q., Jin M., Jiang Y., Huang F., Lou Y, & Zheng L. (2022). One-step synthesized antimicrobial peptide-functionalized gold nanoclusters for selective imaging and killing of pathogenic bacteria. Frontiers in Microbiology, 13, 1003359.

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