Fasting blood samples were collected to measure TC and other laboratory markers, including high-density lipoprotein cholesterol (HDL-C), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), total protein (TP), total bilirubin (TB), blood phosphorus, blood potassium, blood calcium, alkaline phosphatase (ALP), alanine aminotransferase (ALT), aspartate aminotransferase (AST), fasting blood glucose (FBG), and glycated hemoglobin (GH). An Olympus® AU 5400 automated biochemical analyzer (Olympus Corporation, Japan, Shizuoka) was used to assess the blood glucose and lipid levels. Conventional laboratory techniques were used to assess the remaining indicators.
Au5400 automated biochemical analyzer
Manufactured by Olympus
Sourced in Japan
The AU5400 Automated Biochemical Analyzer is a clinical chemistry analyzer manufactured by Olympus. It is designed to perform a wide range of biochemical tests on patient samples. The analyzer uses advanced technology to automate the analysis process, providing efficient and accurate results.
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Lab products found in correlation
4 protocols using au5400 automated biochemical analyzer
1
Blood Biomarker Measurement Protocol
2
Comprehensive Biomarker Analysis in HIV Patients
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CBC tests were performed after an overnight fast and analyzed using a Sysmex SE9000 automated blood cell analyzer. Serum alanine aminotransferase (ALT), albumin, and other serum biochemical parameters were measured using an Olympus AU5400 automated biochemical analyzer. Lymphocyte detection was performed with a flow cytometer. HIV RNA was detected using a polymerase chain reaction machine. Plasma levels of cholesterol and triglycerides (TG) were determined using enzymatic methods. Low-density lipoprotein (LDL)-cholesterol levels were calculated using the Friedewald’s equation. All the detections and evaluations were performed in a central laboratory in the Department of Clinical Laboratory in Nanfang Hospital.
3
Gastric Biomarkers and Metabolic Factors
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The demographic characteristics and clinical variables were collected from the admission record including age, sex, body mass index (BMI), tobacco use and alcohol consumption, systolic blood pressure, diastolic blood pressure, leucocyte count, neutrophil count, lymphocyte count, monocyte count, high-sensitivity C-reactive protein (hs-CRP), blood urea nitrogen (BUN), uric acid (UA), creatinine (Crea), cystatin C (Cysc), total triglycerides (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), homocysteine (Hcy), and medication history. The BMI was computed from measurements of height and weight (kg/m 2 ). Leucocyte count, neutrophil count, lymphocyte count, and monocyte count were measured by the XN-1000 Automated Hematology Analyzer (Sysmex, Japan). The concentration of hs-CRP, BUN, UA, Crea, CysC, TG, TC, HDL-C, LDL-C, and Hcy were measured by the AU5400 Automated Biochemical Analyzer (Olympus, Japan). Serum PG levels were measured by the i2000 using a chemiluminescence immune assay (Abbott, USA), using the manufacturers' reagents, calibrators, and settings. Levels of serum PGI and PGR were categorized according to commonly used cut-off values in gastric diseases [10] , 30 or 70 ng/mL for PGI, and 3 for PGR. PGII lacked of widely-accepted cut-off and was divided into quartiles.
4
Serum Biomarkers for Kidney Injury
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The sera of mice were collected at the indicated time-points. Commercially available blood urea nitrogen (BUN) and serum creatinine (Scr) detection kits (cat. nos. C010 and C074; Changchun Huili Biotech Co., Ltd.) were used to measure serum levels of biochemical parameters including blood urea nitrogen (BUN) and serum creatinine (Scr), according to the manufacturer's instructions, using an Olympus AU5400 Automated Biochemical Analyzer (Olympus Corporation). The serum concentrations of kidney injury molecular-1 (KIM-1) were measured with an ELISA kit (cat. no. EK0880; BOSTER Biological Technology Co., Ltd.) according to the manufacturer's instructions.
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