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Ecl western blotting substrate

Manufactured by Azure Biosystems
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ECL Western Blotting Substrate is a chemiluminescent detection reagent used for the visualization and analysis of proteins in Western blotting experiments. It is designed to provide a sensitive and reliable method for detecting and quantifying target proteins.

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Lab products found in correlation

Iblot 2 dry blotting system, by Thermo Fisher Scientific (2 mentions) Bis tris plus, by Thermo Fisher Scientific (2 mentions) Nanoluc, by Promega (2 mentions) Pierce bca protein assay kit, by Thermo Fisher Scientific (2 mentions) C300 imaging equipment, by Azure Biosystems (2 mentions)

Spelling variants of this product

ECL Substrate (2 citations) Pierce™ ECL Western Blotting Substrate (2 citations)

2 protocols using ecl western blotting substrate

1

Characterization of Extracellular Vesicles

Check if the same lab product or an alternative is used in the 5 most similar protocols
HEK and Nomi EVs were boiled at 95 °C for 10 min and the concentration was determined using Pierce BCA Protein Assay Kit (Thermo Scientific Pierce, Rockford, IL,USA). Then 25 μg of each sample was resolved by electrophoresis on 4–12% SDS-PAGE in Bis-Tris Plus (Thermo Scientific Pierce, Rockford, IL,USA) at 120 V. Next, the proteins were transferred to polyvinylidene fluoride membranes (PVDF) using the Invitrogen iBlot 2 Dry Blotting System. Membranes were blocked by incubation in 5% non-fat milk powder in 0.1% Tween 20 in Tris buffered saline (TBS-T) and incubated overnight at 4 °C with primary antibody against HSP-70 (1:500 Santa Cruz Biotechnology, D1519), Nanoluc (1:500, Promega, N700A), RFP (Thermo Fisher, MA5–15257) at 4 °C overnight. Then, the membranes were washed 3 times with TBS-T for 10 min and incubated with a secondary antibody (1:2500) at RT for 1 h. Membranes were then washed with 3 times TBS-T for 10 min. Bands were visualized by Pierce™ ECL Western Blotting Substrate (32106) in the Azure Biosystems C300 imaging equipment.
Rufino-Ramos D., Lule S., Mahjoum S., Ughetto S., Bragg D.C., de Almeida L.P., Breakefield X.O, & Breyne K. (2022). Using genetically modified extracellular vesicles as a non-invasive strategy to evaluate brain-specific cargo. Biomaterials, 281, 121366.
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2

Characterization of Extracellular Vesicles

Check if the same lab product or an alternative is used in the 5 most similar protocols
HEK and Nomi EVs were boiled at 95 °C for 10 min and the concentration was determined using Pierce BCA Protein Assay Kit (Thermo Scientific Pierce, Rockford, IL,USA). Then 25 μg of each sample was resolved by electrophoresis on 4–12% SDS-PAGE in Bis-Tris Plus (Thermo Scientific Pierce, Rockford, IL,USA) at 120 V. Next, the proteins were transferred to polyvinylidene fluoride membranes (PVDF) using the Invitrogen iBlot 2 Dry Blotting System. Membranes were blocked by incubation in 5% non-fat milk powder in 0.1% Tween 20 in Tris buffered saline (TBS-T) and incubated overnight at 4 °C with primary antibody against HSP-70 (1:500 Santa Cruz Biotechnology, D1519), Nanoluc (1:500, Promega, N700A), RFP (Thermo Fisher, MA5–15257) at 4 °C overnight. Then, the membranes were washed 3 times with TBS-T for 10 min and incubated with a secondary antibody (1:2500) at RT for 1 h. Membranes were then washed with 3 times TBS-T for 10 min. Bands were visualized by Pierce™ ECL Western Blotting Substrate (32106) in the Azure Biosystems C300 imaging equipment.
Rufino-Ramos D., Lule S., Mahjoum S., Ughetto S., Bragg D.C., de Almeida L.P., Breakefield X.O, & Breyne K. (2022). Using genetically modified extracellular vesicles as a non-invasive strategy to evaluate brain-specific cargo. Biomaterials, 281, 121366.
+ Open protocol
+ Expand

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