Col1a1 sc 293182

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Col1a1 (sc-293182) is a laboratory reagent product offered by Santa Cruz Biotechnology. It is an antibody that specifically recognizes the Col1a1 protein, which is a component of type I collagen. The product is intended for use in research applications where detection or quantification of the Col1a1 protein is required.

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COL1A1 (33 citations) Sc-293182 (14 citations)

3 protocols using col1a1 sc 293182

Protein Immunoprecipitation and Western Blot Analysis

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Protein extracts for immunoprecipitation were prepared using nondenaturing immunoprecipitation buffer supplemented with protease and phosphatase inhibitor cocktails (78440, Thermo Fisher Scientific) and 20 mM N-ethylmaleimide (E3876, Sigma-Aldrich). Following protein quantifications, primary antibodies against His-Tag (12698, Cell Signaling Technology), Smad3 (sc-101154, Santa Cruz Biotechnology), or GSH (101-A, Virogen) were added to immunoprecipitate indicated proteins using Protein A Magnetic Beads (S1425S, New England Biolabs). The beads were washed, and protein elutes were analyzed by Western blotting. For Western blotting, cells or homogenized tissues were lysed in radioimmunoprecipitation assay (RIPA) buffer. Primary antibodies for Western blotting include GLRX (ab187507), Smad3 (ab28379), and TGFBR1 (ab235178) from Abcam; α-SMA (A2547) from Sigma-Aldrich; Timp-1 (sc-21734) and Col1a1 (sc-293182) from Santa Cruz Biotechnology; HA-Tag (3724), His-Tag (12698), and p-Smad3 (9520) from Cell Signaling Technology; and ZFYVE9 (PA5-67946) and TGFBR2 (PA5-36115) from Invitrogen. Following incubation with secondary antibodies, Pierce ECL Substrate was used for signal detection.

Xi Y., Li Y., Xu P., Li S., Liu Z., Tung H.C., Cai X., Wang J., Huang H., Wang M., Xu M., Ren S., Li S., Zhang M., Lee Y.J., Huang L., Yang D., He J., Huang Z, & Xie W. (2021). The anti-fibrotic drug pirfenidone inhibits liver fibrosis by targeting the small oxidoreductase glutaredoxin-1. Science Advances, 7(36), eabg9241.

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Collagen Type I Immunoblotting Protocol

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GA and aminoguanidine (AG) were purchased from Nacalai Tesque (Kyoto, Japan) and Wako (Osaka, Japan)., respectively. Anti-collagen type 1 α 1 (COL1A1; sc-293182) and anti-β-tubulin (014-25041) antibodies were from Santa Cruz (CA, USA) and Wako, respectively. A horseradish peroxidase (HRP)-conjugated secondary antibody was purchased from Cell Signaling Technology (MA, USA). An anti-TAGE antibody was prepared and purified as previously described [18 (link)].

Sakasai-Sakai A., Takata T, & Takeuchi M. (2022). The Association between Accumulation of Toxic Advanced Glycation End-Products and Cytotoxic Effect in MC3T3-E1 Cells. Nutrients, 14(5), 990.

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Western Blot Analysis of Osteogenic Markers

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RNA immunoprecipitation assay lysis buffer (Beyotime, Shanghai, China) was used for extracting proteins from femur tissues and human osteoblasts. The protein concentrations were assessed by a bicinchoninic acid protein assay kit (Beyotime, China), and then received subjection to 10% sodium dodecyl sulfate, polyacrylamide gel electrophoresis gel and shifted onto polyvinylidene difluoride membranes (Millipore, USA). Membranes were then sealed with 5% nonfat milk, followed by treatment with the primary antibodies at 4℃ overnight. The primary antibodies were: COL1A1 (sc-293182, Santa Cruz Biotechnology), COL1A2 (sc-393573, Santa Cruz Biotechnology), ALP (ab224335, Abcam), OCN (ab133612, Abcam), TGF-β (ab215715, Abcam), Smad3 (ab40854, Abcam), p-Smad3 (ab51177, Abcam), Smad2 (ab40855, Abcam), p-Smad2 (ab188334, Abcam), BMP2 (ab214821, Abcam) and β-actin (ab6276, Abcam). Next, the membranes were cultured with secondary antibodies at room temperature for 1 h. β-actin acted to be the internal control. An enhanced chemiluminescence detection kit (Thermo Fisher Scientific) was adopted for visualizing protein bands and the ImageJ software was used for densitometry analysis of the band intensity.

Wang S., Xu B., Yin H., Hua Z., Shao Y, & Wang J. (2024). Ginsenoside Rc alleviates osteoporosis by the TGF-β/Smad signaling pathway. Cellular and molecular biology (Noisy-le-Grand, France), 70(3).

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