Fmk001

Manufactured by Merck Group

FMK001 is a laboratory instrument designed for conducting various scientific experiments and analyses. It offers precise and reliable performance for a range of applications. The core function of FMK001 is to provide accurate and consistent data to support research and development activities.

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Lab products found in correlation

Vincristine, by Merck Group (1 mentions) Anti xpress, by Thermo Fisher Scientific (1 mentions) Bafilomycin, by Merck Group (1 mentions) Anti gapdh, by Santa Cruz Biotechnology (1 mentions) Anti lc3a b, by Cell Signaling Technology (1 mentions) Anti alpha tubulin, by Santa Cruz Biotechnology (1 mentions) D8418 250ml, by Merck Group (1 mentions) Anti beta 3 tubulin, by Abcam (1 mentions) Mg132, by Merck Group (1 mentions) Anti ha, by Abcam (1 mentions) Anti flag, by Cell Signaling Technology (1 mentions) Anti gfp, by Santa Cruz Biotechnology (1 mentions) Anti gst, by Santa Cruz Biotechnology (1 mentions) Anti sumo2 3, by Abcam (1 mentions) T8787, by Merck Group (1 mentions) Palmitate, by Merck Group (1 mentions) Gkt137831, by Cayman Chemical (1 mentions) Paraformaldehyde (pfa), by Thermo Fisher Scientific (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions) Phorbol myristate acetate (pma), by Merck Group (1 mentions)

2 protocols using fmk001

Antibody-Based Protein Interaction Analysis

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The following antibodies were used: anti-GFP (Santa Cruz, sc-9996 for coimmunoprecipitation; Abcam, ab32146 for immunoblots), anti-FLAG horseradish peroxidase-conjugated (Sigma, A8592), anti-p-SEK1/MKK4 (Cell Signaling, CST-9151), anti-GST (Santa Cruz, sc-138), anti-alpha-tubulin (Santa Cruz, sc-53030), anti-DLK (ThermoFisher Scientific, PA5-32173 for coimmunoprecipitation and immunohistochemistry; Antibodies Incorporated, 75-355 for immunoblot), anti-GAPDH (Santa Cruz, sc-32233), anti-LC3A/B (Cell Signaling, CST-12741), anti-HA (Abcam, ab9110), anti-Xpress (ThermoFisher Scientific, R910-25), anti-SUMO2/3 (Abcam, ab3742), anti-FLAG (Cell Signaling, 14793S), anti-BRN3A Alexa Fluor 594 (Santa Cruz, sc-8429 AF594), and anti-beta III tubulin (Abcam, ab41489). We dissolved all chemicals in dimethyl sulfoxide (Sigma, D8418-250ML) except for vincristine (Sigma, V8879), which was dissolved in methanol. Controls were treated with dimethyl sulfoxide as vehicle or methanol in the case of vincristine controls. We used vincristine at 200 nM, bafilomycin (Sigma, B1793) at 100 nM, caspase inhibitor (Sigma, 400012) at 1 and 5 μM, pan-caspase inhibitor (R&D systems, FMK001) at 10 and 50 μM, and MG-132 (Sigma, M7449) at 10 μM.

Lee B., Oh Y., Cho E., DiAntonio A., Cavalli V., Shin J.E., Choi H.W, & Cho Y. (2022). FK506-binding protein-like and FK506-binding protein 8 regulate dual leucine zipper kinase degradation and neuronal responses to axon injury. The Journal of Biological Chemistry, 298(3), 101647.

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Immunocytochemistry of Cellular Activation

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4′,6-diamidino-2-phenylindole (DAPI, D1306, Thermo Fisher), diphenyleneiodonium chloride (DPI, 141310, Abcam), GKT-137831 (17764, Cayman Chemical), palmitate (P5585, Sigma), paraformaldehyde (PFA, 28908; Thermo Fisher), phorbol 12-myristate 13-acetate (PMA, P1585, Sigma), Triton X-100 (T8787, Sigma), and Z-VAD-FMK (FMK001, Sigma).

Dong J., Viswanathan S., Adami E., Singh B.K., Chothani S.P., Ng B., Lim W.W., Zhou J., Tripathi M., Ko N.S., Shekeran S.G., Tan J., Lim S.Y., Wang M., Lio P.M., Yen P.M., Schafer S., Cook S.A, & Widjaja A.A. (2021). Hepatocyte-specific IL11 cis-signaling drives lipotoxicity and underlies the transition from NAFLD to NASH. Nature Communications, 12, 66.

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