E15.5 embryos were processed whole, while E18.5 embryos were skinned and trisected to capture the region below the mandible and above the abdomen. Samples were fixed in Bouin’s fixative (Sigma, HT101128) at room temperature and graded into EtOH, histoclear (National Diagnostics, HS-200), and paraffin before being embedded in paraffin. Coronal serial sections were cut at a thickness of 7 μm and subsequently stained with hematoxylin and eosin. Imaging was performed on a Zeiss Imager.Z2 and 3D reconstructed models were produced using Avizo (ThermoFisher): sections were aligned using the “align slices” function, lumens of the pharynx, larynx, trachea, bronchi, and esophagus model labels were selected by thresholding for unstained regions and selecting with the “magic wand” tool within the segmentation pane, model labels were processed using the “resample” tool with “voxel averaging” of “3 × 3 × 1”, a surface model was generating using “generate surface” with “constrained smoothing” of “extent” 2, and models were visualized with the “surface view” module.
Hs 200
Manufactured by National Diagnostics
The HS-200 is a compact and automated hematology analyzer designed for routine blood cell analysis. It provides comprehensive blood cell counts and differentiation across a range of parameters. The HS-200 is a reliable and efficient solution for clinical laboratories and healthcare settings.
Automatically generated - may contain errors
3 protocols using hs 200
1
Embryonic Airway Reconstruction from Histological Sections
2
Carmine Alum Whole Mount Staining
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The 4th pair of mammary glands were dissected and fixed for 2 hr in 4% paraformaldehyde, and then washed the tissue three times in PBS for 15 min each time. Finally, the tissues were stained in carmine alum solution (2 mg/ml carmine [Sigma; C1022], 5 mg/ml KAl(SO4)2 in H2O) overnight at room temperature. After the staining, the tissues were washed in de-staining solution (50% ethanol, 2% HCl) for 2 hr, and then serial dehydrated in 75%, 85%, 95%, 100%, 100% ethanol and finally stored in Histoclear (National Diagnostics; HS-200). Whole mount analyses were performed under a dissection microscope (Leica).
3
Embryonic Airway Reconstruction from Histological Sections
Check if the same lab product or an alternative is used in the 5 most similar protocols
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E15.5 embryos were processed whole, while E18.5 embryos were skinned and trisected to capture the region below the mandible and above the abdomen. Samples were fixed in Bouin’s fixative (Sigma, HT101128) at room temperature and graded into EtOH, histoclear (National Diagnostics, HS-200), and paraffin before being embedded in paraffin. Coronal serial sections were cut at a thickness of 7 μm and subsequently stained with hematoxylin and eosin. Imaging was performed on a Zeiss Imager.Z2 and 3D reconstructed models were produced using Avizo (ThermoFisher): sections were aligned using the “align slices” function, lumens of the pharynx, larynx, trachea, bronchi, and esophagus model labels were selected by thresholding for unstained regions and selecting with the “magic wand” tool within the segmentation pane, model labels were processed using the “resample” tool with “voxel averaging” of “3 × 3 × 1”, a surface model was generating using “generate surface” with “constrained smoothing” of “extent” 2, and models were visualized with the “surface view” module.
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