Acetonitrile c2h3n

DNA probes (aptamer sequence: 5′-ACA CGA CGA GGG ACG AGG AGT ACT TGC CAA CGA TAA CGT CGT TGG ATC TGT CTG TGC CC-3′) were purchased from Sangon Biotechnology Co., Ltd. (Shanghai, China). HAuCl₄·H₂O was purchased from Beijing Chemical Reagent Company (Beijing, China). Dexamethasone was purchased from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China). Trisodium citrate dihydrate was obtained from Yongsheng Fine Chemicals Company (Tianjin, China). Methanol (CH₃OH) and Acetonitrile (C₂H₃N) were obtained from Fisher Scientific Co., Ltd. (Shanghai, China). Ethyl acetate(C₄H₈O₂) was purchased from Tianjin Beilian Fine Chemicals Development Co., Ltd. (Tianjin, China). Milk and glucosamine samples were obtained at the local market. All experiments were repeated three times, and the standard deviation (error bars) on three sets of measurements were calculated by Origin software.

Analytical-grade salts, buffer reagents, chloramine T, hydrochloric acid (HCl), acetic acid (CH₃COOH), potassium iodide (KI), methanesulfonic acid (CH₄O₃S), adrenaline, glutathione reductase (GR), peroxidase, and ethylenediaminetetraacetic acid (EDTA) were purchased from Sigma-Aldrich, St. Louis, MO, USA. 3,3′,5,5′-tetramethylbenzidine (TMB), nicotine adenine dinucleotide phosphate (NADPH), L glutathione reduced (GSH), and oxidized (GSSG) were from SERVA Electrophoresis GmbH, Heidelberg, Germany. Hydrogen peroxide (H₂O₂), methanol, and 2,5-dihydroxybenzoic acid (DHB) were provided by Merck KGaA, Darmstadt, Germany. 5,5′-dithio-bis-2-nitrobenzoic acid (DTNB) was purchased from Acros Organics, Geel, Belgium, while 1-methyl-2-phenylindole (MFI) and uric acid were from Alfa Aesar, Ward Hill, MA, USA. Dimethyl sulfoxide (DMSO), chloroform, and acetonitrile (C₂H₃N) were from Fisher Scientific, Waltham, MA, USA.