Acetanilide c8h9no

Manufactured by Merck Group

Sourced in Germany

Acetanilide (C8H9NO) is a chemical compound used as a laboratory reagent. It has the molecular formula C8H9NO and a molar mass of 135.18 g/mol. Acetanilide is a crystalline solid at room temperature.

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Na 1500, by Carlo Erba (1 mentions) Delta 5 plus, by Thermo Fisher Scientific (1 mentions) Euro ea 3000, by Eurovector (1 mentions)

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Acetanilide (22 citations)

3 protocols using acetanilide c8h9no

Stable Isotope Analysis of Oak Microcuttings and Collembola

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For ¹³C and ¹⁵N analysis of the compartments of oak microcuttings, the plant material was dried, milled, weighed into tin capsules (1.50–1.75 mg) and stored in a desiccator until analysis (plant material contained pools (leaves and lateral roots) or individual plants (stems and principal roots); for pools see electronic supplementary material, table S2). For ¹³C and ¹⁵N analysis of Collembola, 15–20 individuals of P. armata, equivalent to 50–200 µg dry weight, were transferred into tin capsules, dried at 60°C for 24 h and stored in a desiccator until analysis.
Samples were analysed with a combined system consisting of an elemental analyser (NA 1500, Carlo Erba, Milan, Italy) and a mass spectrometer (MAT 251, Finnigan, Bremen, Germany) [39 ]. The precision of the measurement is 0.1 delta per mil for ¹³C and 0.2 delta per mil for ¹⁵N. Stable isotope abundance is expressed as atom% excess calculated as ¹³C (%) = [¹³C/(¹²C + ¹³C)]/100 and ¹⁵N (%) = [¹⁵N/(¹⁴N + ¹⁵N)]/100. For ¹³C PD belemnite (PDB) and for ¹⁵N atmospheric N was used as primary standard. Acetanilide (C₈H₉NO; Merck, Darmstadt, Germany) was used for internal calibration.

Graf M., Bönn M., Feldhahn L., Kurth F., Grams T.E., Herrmann S., Tarkka M., Buscot F, & Scheu S. (2019). Collembola interact with mycorrhizal fungi in modifying oak morphology, C and N incorporation and transcriptomics. Royal Society Open Science, 6(3), 181869.

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Stable Isotope Analysis of Terrestrial Organisms

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Larvae and adults of gnats and midges, plant- and leafhoppers, spiders and rice plants were dried at 60 °C for 48 h. Large spiders such as tetragnathids (Tetragnathidae) were ground entirely and a subsample used for the analysis; while small species were used whole. Samples were transferred into tin capsules which were closed before analysis. SIA was carried out by a combination of an elemental analyser (NA 1110, CA-Instruments, Milano, Italy) coupled with an isotope mass spectrometer (Delta Plus, Finnigan MAT, Bremen, Germany; Reineking et al. 1993 (link)). Natural variations in stable isotope ratios were expressed using the δ notation as δX (‰) = (R_sample − R_standard)/R_standard × 1000, with X representing ¹³C or ¹⁵N, R_sample the ¹³C/¹²C or ¹⁵N/¹⁴N ratio of the sample and R_standard the respective ratios of the standard. Vienna Peedee Belemnite limestone and atmospheric nitrogen were used as standards for ¹³C and ¹⁵N, respectively. Acetanilide (C₈H₉NO, Merck, Darmstadt) was used for internal calibration.

Radermacher N., Hartke T.R., Villareal S, & Scheu S. (2020). Spiders in rice-paddy ecosystems shift from aquatic to terrestrial prey and use carbon pools of different origin. Oecologia, 192(3), 801-812.

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Stable Isotope Analysis of Soil Biota

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Specimens were transferred into tin capsules. Rare (e.g. Carabodes willmanni) or smallersized species (e.g. Microppia minus) required the pooling of several individuals to reach the biomass necessary to the analysis. After drying at 60°C for at least 12 h, samples were reweighed and stored in a desiccator until further analysis. The same procedure was used to prepare samples of nematodes, extracted from fresh soil by using a modified Baermann funnel method. Soil, mosses, lichens, roots, and plant material were ground and subjected to the same procedure (root and plant material 1.0 -1.5 mg, soil 34.1 -35.3 mg). We analysed these organisms and material to obtain baseline values of different potential food sources for oribatid mites (Supplementary Material). A coupled system of an elemental analyzer (Euro EA 3000, Euro Vector S.p.A.: Milano, Italy) and a mass spectrometer (Delta V Plus Thermo Electron; Bremen, Germany) was used to analyze the 13 C/ 12 C and nitrogen whereas acetanilide (C8H9NO, Merck, Darmstadt, Germany) served for internal calibration. Vienna Pee Dee Belemnite (V-PDB) was used as a primary standard for 13 C.
See also Fischer et al. (2010) (link), Maraun et al. (2011 (link)), Pollierer et al. (2009 (link)), and Schneider et al. (2004) (link) for further details.

Maaß S., Maraun M., Scheu S., Rillig M.C, & Caruso T. (2015). Environmental filtering vs. resource-based niche partitioning in diverse soil animal assemblages. Soil biology & biochemistry, 85.

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